Figure 2 The immunophenotype characteristics of the DPSCs and iDPSCs. (A) Immunophenotype analysis by flow cytometry. The third-passage cells were incubated with monoclonal antibodies against the cell-surface marker antigens CD29, CD90, CD166, CD34, and CD45, followed by fluorescein-conjugated secondary antibodies. Both DPSCs and iDPSCs showed similar cell-surface marker expressions. (B) The percentage of positivity of the markers were calculated from 5 samples. (C) Immunofluorescence staining. The nuclei were stained with DAPI (blue). Both DPSCs and iDPSCs co-expressed CD166 and CD90 (magnification, ×20; Scale bar, 100 μm). DPSCs – dental pulp stem cells; iDPSCs – inflammatory dental pulp stem cells; MSCs – mesenchymal stem cells; DAPI – 4′,6-diamidino-2-phenylindole. (Figure were created using Adobe Photoshop CS3, Adobe Systems Software Ireland, Ltd.).