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18 March 2022: Clinical Research

Unveiling the Differences in Biological Properties of Dental Pulp Stem Cells from Normal and Inflamed Pulp: A Comprehensive Comparative Study

Shao-Chen Nie 12ABCDEF* , Kun Yang 3ABCD* , Na-Na Luan 12F , Xiao-Li Lian 2B , Xiao-Hua Dai 2C , Su-Xia Liang 12A* , Ying-Bin Yan 24A*

DOI: 10.12659/MSM.934511

Med Sci Monit 2022; 28:e934511

Figure 2 The immunophenotype characteristics of the DPSCs and iDPSCs. (A) Immunophenotype analysis by flow cytometry. The third-passage cells were incubated with monoclonal antibodies against the cell-surface marker antigens CD29, CD90, CD166, CD34, and CD45, followed by fluorescein-conjugated secondary antibodies. Both DPSCs and iDPSCs showed similar cell-surface marker expressions. (B) The percentage of positivity of the markers were calculated from 5 samples. (C) Immunofluorescence staining. The nuclei were stained with DAPI (blue). Both DPSCs and iDPSCs co-expressed CD166 and CD90 (magnification, ×20; Scale bar, 100 μm). DPSCs – dental pulp stem cells; iDPSCs – inflammatory dental pulp stem cells; MSCs – mesenchymal stem cells; DAPI – 4′,6-diamidino-2-phenylindole. (Figure were created using Adobe Photoshop CS3, Adobe Systems Software Ireland, Ltd.).

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750