18 March 2022 : Clinical Research
Unveiling the Differences in Biological Properties of Dental Pulp Stem Cells from Normal and Inflamed Pulp: A Comprehensive Comparative Study
Shao-Chen Nie12ABCDEF, Kun Yang3ABCD, Na-Na Luan12F, Xiao-Li Lian2B, Xiao-Hua Dai2C, Su-Xia Liang12A*, Ying-Bin Yan24ADOI: 10.12659/MSM.934511
Med Sci Monit 2022; 28:e934511
Figure 4 Osteogenic/dentinogenic differentiation induction analysis. (A) Mineralized nodules were detected after 14 days of induction following alizarin red (AR) staining. “+”, inductive medium; “−”, control medium. Scale bar, 100 μm. (B) The bar chart represents the percentage of area positively stained for AR (mean±standard deviation) analyzed from 5 samples, * P<0.05. (C, D) Several osteogenic/dentinogenic lineage-specific genes were examined by quantitative real-time polymerase chain reaction after 1-week and 2-week induction, * P<0.05. DPSCs – dental pulp stem cells; iDPSCs – inflammatory dental pulp stem cells. (Figure were created using Adobe Photoshop CS3, Adobe Systems Software Ireland, Ltd.).