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18 March 2022: Clinical Research

Unveiling the Differences in Biological Properties of Dental Pulp Stem Cells from Normal and Inflamed Pulp: A Comprehensive Comparative Study

Shao-Chen Nie 12ABCDEF* , Kun Yang 3ABCD* , Na-Na Luan 12F , Xiao-Li Lian 2B , Xiao-Hua Dai 2C , Su-Xia Liang 12A* , Ying-Bin Yan 24A*

DOI: 10.12659/MSM.934511

Med Sci Monit 2022; 28:e934511

Figure 6 Expression of nestin and tubulin in DPSCs and iDPSCs. The cells were cultured in non-differentiated control medium (−) and neurogenic differentiation medium (+) for 48 hours. (A) Non-stimulated cells demonstrated the absence or much weaker staining for nestin and tubulin than stimulated cells (immunohistochemistry staining, magnification, ×40; scale bar, 50 μm). (B) Immunofluorescence staining of DPSCs and iDPSCs. All nuclei were stained with DAPI (blue). The cells were stained with antibody to nestin (green), and tubulin (red). Magnification, ×20; scale bar, 100 μm. (C) Quantitative real-time polymerase chain reaction measurements for neurogenic markers nestin and tubulin. The mRNA levels of 5 independent samples (n=5) were measured. * P<0.05. DPSCs – dental pulp stem cells; iDPSCs – inflammatory dental pulp stem cells; DAPI – 4′,6-diamidino-2-phenylindole. (Figure were created using Adobe Photoshop CS3, Adobe Systems Software Ireland, Ltd.).

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750