Figure 6 Expression of nestin and tubulin in DPSCs and iDPSCs. The cells were cultured in non-differentiated control medium (−) and neurogenic differentiation medium (+) for 48 hours. (A) Non-stimulated cells demonstrated the absence or much weaker staining for nestin and tubulin than stimulated cells (immunohistochemistry staining, magnification, ×40; scale bar, 50 μm). (B) Immunofluorescence staining of DPSCs and iDPSCs. All nuclei were stained with DAPI (blue). The cells were stained with antibody to nestin (green), and tubulin (red). Magnification, ×20; scale bar, 100 μm. (C) Quantitative real-time polymerase chain reaction measurements for neurogenic markers nestin and tubulin. The mRNA levels of 5 independent samples (n=5) were measured. * P<0.05. DPSCs – dental pulp stem cells; iDPSCs – inflammatory dental pulp stem cells; DAPI – 4′,6-diamidino-2-phenylindole. (Figure were created using Adobe Photoshop CS3, Adobe Systems Software Ireland, Ltd.).