Figure 5 Effect of BNIP3 on cell viability and apoptosis in IH-induced HK-2 cellsAfter being transfected with siBNIP3 and BNIP3 vector, HK-2 cells were exposed to IH for 24 h. Cell morphology was assessed by microscopy and cell viability was quantitatively determined with CCK-8 (A). Flow cytometry (B) and TUNEL staining (C) were conducted to detect cell apoptosis. The figure was created using a Hitachi electron microscope version H7700 (Hitachi, Tokyo, Japan) and Confocal laser scanning microscopy version E-C1 (Nikon, Tokyo, Japan), and GraphPad Prism version 5.0 (GraphPad Software, Inc., LaJolla, CA, USA).