Figure 4 Knockdown of NFYAv1 impaired HCC cell growth and cell cycle progression(A) A schematic chart showing the strategy used to perform single-gene GSEA and a summary of the gene sets associated with high NFYAv1 expression. (B) GSEA plots showing the graphical view of the enrichment score for the mitotic spindle and G2/M checkpoint gene sets associated with high NFYAv1 expression. (C) RT-qPCR (upper panel) and western blot (bottom panel) assays were performed to detect the expression of NFYA at the mRNA and protein levels in SK-Hep-1 and Hep3B cells 48 h after infection with lentiviral shNFYA. (D, E) CCK-8 assays were conducted to measure the viability of SK-Hep-1 and Hep3B cells with or without NFYA knockdown. (F–H) Cell cycle analysis was performed through PI staining and following flow cytometry for SK-Hep-1 (F upper panel and G) and Hep3B (F bottom panel and G) cells after lentiviral shNFYA infection. Quantitative measurements (F, G) of the cell cycle phase were conducted. Data are mean±SD (n=3) of 3 biological repeats. **, P<0.01; ***, P<0.001.