14 June 2023 : Clinical Research
Influence of Umbilical Cord Blood Biochemical Parameters and Disease Condition on the Expression of the TSG-6 Gene in Umbilical Mesenchymal Stem Cells
Aleksandra Ozygała1ABDEFG, Gabriela Wojciechowska1BDEF, Kinga Traczyk1BDEF, Dominika Przywara23ABDEF, Alicja Petniak
2BDEF, Rafał Szymanowski2BE, Agnieszka Wilińska2BE, Arkadiusz Krzyżanowski4BF, Anna Kwaśniewska4BF, Bartosz J. Płachno5EF, Paulina Gil-Kulik 6ABCDEF*, Janusz Kocki2ADEG
DOI: 10.12659/MSM.939716
Med Sci Monit 2023; 29:e939716
Figure 2 Cytometric evaluation of the expression of CD146, CD73, CD90, and CD105 surface antigens on the tested cells. DuraClone SC Mesenchymal Tube antibodies, Navios cytometer (Beckman Coulter). Sample immunophenotype analysis of umbilical cord-derived mesenchymal stem cells (MSCs) after in vitro culture. (A) Cytogram: cell population negative for CD45 antigen (CD45−). (B) Cytogram: percentage of CD31-negative and CD146-positive cells (CD146+CD31−). (C) Cytogram: percentage of cell population expressing CD90 antigen and CD73 antigen (CD90+CD73+). (D) Cytogram: percentage of umbilical cord MSCs positive for CD90, CD105, CD73 (CD90+CD105+CD73+). (E) MSC positive for CD90, CD105, CD73 and negative for CD14 and CD19 (CD90+CD105+CD73+CD14−CD19−).