14 June 2023: Clinical Research
Influence of Umbilical Cord Blood Biochemical Parameters and Disease Condition on the Expression of the Gene in Umbilical Mesenchymal Stem Cells
Aleksandra Ozygała 1ABDEFG , Gabriela Wojciechowska 1BDEF , Kinga Traczyk 1BDEF , Dominika Przywara 23ABDEF , Alicja Petniak 2BDEF , Rafał Szymanowski 2BE , Agnieszka Wilińska 2BE , Arkadiusz Krzyżanowski 4BF , Anna Kwaśniewska 4BF , Bartosz J. Płachno 5EF , Paulina Gil-Kulik 2ABCDEF** , Janusz Kocki 2ADEG*DOI: 10.12659/MSM.939716
Med Sci Monit 2023; 29:e939716
Figure 2 Cytometric evaluation of the expression of CD146, CD73, CD90, and CD105 surface antigens on the tested cells. DuraClone SC Mesenchymal Tube antibodies, Navios cytometer (Beckman Coulter). Sample immunophenotype analysis of umbilical cord-derived mesenchymal stem cells (MSCs) after in vitro culture. (A) Cytogram: cell population negative for CD45 antigen (CD45−). (B) Cytogram: percentage of CD31-negative and CD146-positive cells (CD146+CD31−). (C) Cytogram: percentage of cell population expressing CD90 antigen and CD73 antigen (CD90+CD73+). (D) Cytogram: percentage of umbilical cord MSCs positive for CD90, CD105, CD73 (CD90+CD105+CD73+). (E) MSC positive for CD90, CD105, CD73 and negative for CD14 and CD19 (CD90+CD105+CD73+CD14−CD19−).