Figure 2 Weighted gene co-expression network analysis (WGCNA) co-expression network of immune-related genes. (A) The optimal soft-threshold power. Analysis of soft-thresholding powers to fit the scale-free topology model and the mean connectivity of the soft-thresholding powers; a scale-free topological criterion >0.8, 9 was chosen as the value to construct a scale-free network. (B) Dendrogram based on a dissimilarity metric for differentially expressed genes. The branches represent different gene modules, and each leaf represents a gene in the cluster dendrogram. (C) Module-trait relationships between WGCNA modules and immune cells. (D) Scatter plots of key modules. The significant correlations between gene significance (GS) for macrophages/M1 macrophages and module membership (MM) were presented in the yellow module. (R version 4.2.1.).