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26 January 2024: Lab/In Vitro Research

CTRP13 Mitigates Endothelial Cell Ferroptosis via the AMPK/KLF4 Pathway: Implications for Atherosclerosis Protection

Jie Du 123ABCDEF , Jianjun Wu 12ABC , Youqi Zhang 12CDF , Qi Liu 12CDF , Xing Luo 12ABD , Xingtao Huang 12ABC , Xuedong Wang 12ABC , Fan Yang 12AEF , JingBo Hou 12ABCDEFG*

DOI: 10.12659/MSM.942733

Med Sci Monit 2024; 30:e942733

Figure 2 C1q/tumor necrosis factor-related protein 13 (CTRP13) suppressed oxidized low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cell (HUVEC) ferroptosis. (A) Quantification of HUVEC viability following different doses of CTRP13 treatment. (B) Quantification of HUVEC viability following different doses of CTRP13 treatment following co-incubation with or without ox-LDL 100 μg/mL. (C–E) Acyl-CoA synthetase long-chain family member 4 (ACSL4), glutathione peroxidase 4 (GPX4), and recombinant solute carrier family 7, member 11 (SLC7A11) mRNA expressions. (F) Protein expression levels were measured using Western blot analysis. The following groups were assessed: Control; ox-LDL; ox-LDL+CTRP13150 ng/mL; ox-LDL+CTRP13 300 ng/mL; ox-LDL+CTRP13 450 ng/mL); ox-LDL+Fer-1, HUVECs were pre-treated with ox-LDL 100 μg/mL. (G–I) Quantitative analysis of ferroptosis-associated protein expression. The data are expressed as the mean±SD (n=3–4 per group). Statistical power=0.953772, effect size(r)=0.858954. * P<0.05 vs the control group. # P<0.05 vs the ox-LDL group. The relative protein levels were quantified by Image J software (NIH, Bethesda, MD, USA). GraphPad Prism 9.0 software (La Jolla, USA) was used to analyze the data.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750