Figure 3 Lipid metabolic alterations in patients with psoriasis after methotrexate (MTX) treatment(A) Principal component analysis plot clearly separating samples from 0 and 12 weeks of MTX treatment in cohort 1. (B) Volcano plot showing 139 differentially expressed metabolites. (C) Three representative lipids were significantly altered after 12 weeks of MTX treatment. Each dot indicates an individual. The 1 quantile and mean and 3 quantile levels are shown in the boxplot. (D) Metabolic pathways showed that the differentially expressed lipids are prominent in the glycerophospholipid pathway. (Figures were generated by R with ggplot2_3.4.2, EnhancedVolcano_1.16.0. The metabolic pathways analysis was based on MetaboAnalyst 6.0.).