Figure 6 Knockdown of MRTO4 negatively regulated ALDOB to inhibit glycolysis. (A) Heatmap showing the top 35 genes in liver cancer that were positively and negatively related to MRTO4. Red represents positively related genes and blue represents negatively related genes. (B) Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of MRTO4-related genes in HCC. (C) RT-qPCR assayed the expression of ALDOB. (D) Immunofluorescence analyzed the expression levels of ALDOB in the cells. (E) Protein imprint measured ALDOB expression in HCC cells. (F) Seahorse extracellular flux analyzer assessed the ECAR of HepG2 and MHCC97H. (G) ELISA assessed the cells for glucose consumption. (H) ELISA assessed the cellular lactate production capacity. (I) ELISA assessed the level of ATP released from the cells. All results are presented as mean±SD (n=3).* P<0.05, ** P<0.01, *** P<0.001.