Figure 3 Changes in regions of interest (ROI) 3–4, dynamic lung compliance (Cdyn), mean velocity of blood flow in middle cerebral artery (Vm), cerebral oxygen saturation (rScO2), heart rate (HR), and mean arterial pressure (MAP) in manual sustained inflation (SI) group and a stepwise increase in positive end-expiratory pressure (IP) group at baseline, peak airway pressure (Ppeak)=30cmH2O, 1 min after recruitment maneuvers (RM), 10 min after RM and end of surgery. (A) Changes of ROI 3–4 comparison. (B) Changes of Cdyn comparison. (C) Changes of Vm comparison. (D) Changes of rScO2 comparison. (E) Changes of HR comparison. (F) Changes of MAP comparison. * P<0.05 vs baseline within group. # P<0.05 SI Group vs IP Group. (GraphPad Prism, version 8.0.2, GraphPad Software, California, USA).