Figure 2 Phenotype of CD4+ T cell population. CD4+ cells (A, C, E, G) and/or PBMCs (B, D, F, H) were co-cultured for 5 days with ASCs or ASCsTI obtained from patients with OA (n=24) or patients with RA (n=24). Monocultures of T cells (T) or PBMCs served as controls. Percentages of CD4+ T cells expressing surface protein or transcription factor are presented: CD69 (A, B), CD25 (C, D), HLA-DR (E, F), CD25 and FoxP3 (G, H). Data are expressed as the median (horizontal line) with interquartile range (IQR, box), lower and upper whiskers (data within 3/2xIQR), and outliers (points) (Tukey’s box). The p values are expressed as follows: * 0.05>p>0.01; ** 0.01>p>0.001); *** p<0.001. ASCsTI – ASCs pretreated with TI. Figure created using GraphPad Prism software version 7, Boston, MA, USA.