Figure 8 Production of TNF and IFNγ. Concentrations of TNF (A–D) and IFNγ (E–H) detected in the culture supernatants of OA-derived (A, B, E, F, n=16) or RA-derived (C, D, G, H, n=14) ASCs or ASCsTI with PBMCs. Monocultures of PBMCs served as controls. The used solutions were: 1-MT, indomethacin, αTGF-β. Every solution was used with RA- and OA-derived ASCs or ASCsT. Results are shown as single values (box plots) with a standard deviation (vertical lines) and median (horizontal line). The p values for comparisons of co-cultures versus control monocultures are as follows: * 0.05>p>0.01; ** 0.01>p>0.001; *** p<0.001; **** p<0.0001. ASCsTI – ASCs pretreated with TI. Figure created using GraphPad Prism software version 7, Boston, MA, USA.