04 November 2024 : Animal Research
Impact of Fresh Khat Extract on Proinflammatory Cytokines and Hepatic and Renal Responses
Siddig I. Abdelwahab1ABCDEFG*, Manal M.E. Taha
1ABCE, Sivakumar S. Sivagurunathan Moni 2BC, Mahdi M. Alnajai 3B, Ahmed A. Jerah3BEG, Abdullah Farasani4BEG, Saleh M. Abdullah4BEG, Ieman A. Aljahdali5BEG, Omar Oraibi 6BEG, Bassem Oraibi1BEG, Hassan A. Alfaifi7BEG, Amal H. Alzahrani8BEG, Yasir O.H. Babiker 9BEG, Adel S. Al-Zubairi 1011BE
DOI: 10.12659/MSM.946108
Med Sci Monit 2024; 30:e946108
Figure 5 Quantitative assessment of TNF-α levels in serum using sandwich ELISA. Serum samples were collected and analyzed using TNF-α rat ELISA kits from MyBioSource (USA). The optical density was measured at 450 nm with an ELISA reader (ELx800, USA). TNF-α levels were calculated based on a standard curve and expressed in pg/mL. The figure provides an overview of the experimental procedure and represents the quantitative assessment of TNF-α levels in serum. * Indicates statistically significant differences; ns – no significant changes. (A) Female groups; (B) male groups; (C) comparison between male and female groups. (This figure was generate using GraphPad Prism version 9, GraphPad Software, San Diego, California, USA).