Figure 4 Paired analysis of immunological parameters in patients with secondary hemophagocytic lymphohistiocytosis (sHLH) patients before and after achieving complete response (CR). Longitudinal data from 6 sHLH patients who achieved CR are shown, comparing immunological markers at initial diagnosis (sHLH) and post-treatment (CRsHLH). Each line connects the measurements from a single patient at the 2 time points. The analysis includes proportions of CD8+ T cells, NK cells, and CD56+ T cells (A), as well as the percentage of perforin-expressing cells within each of these subsets (B). A significant decrease was observed in the proportion of CD8+ T cells following treatment (P=0.0028). No other parameters showed statistically significant changes (P>0.05). Statistical significance was determined using the Wilcoxon signed-rank test for paired samples. Figure created with GraphPad Prism v8.0.