05 May 2026 : Animal Research
RAPTOR Silencing Inhibits Head and Neck Squamous Cell Carcinoma Progression via Regulation of S100A8/A9 and Urokinase Plasminogen Activator
Zhenzhen Fu BCDEF 1,2, Song Wang BCDEF 3, Haoran Zheng BCDEF 2, Huadong Wu BCD 2, Tao Li BCF 2, Lihao Wang BC 2, Hong Li BCF 2, Qiang Zhang AEG 2*DOI: 10.12659/MSM.952169
Med Sci Monit 2026; 32:e952169
Figure 10 Rapamycin inhibits HNSCC cell aggressiveness in vitro and tumor growth in vivo. (A) CCK-8 assays showing decreased proliferation in Cal27, HSC3, and HN30 cells after 48 h of rapamycin treatment. (B–D) Colony formation, wound-healing, and Transwell assays were conducted using 50 nM rapamycin, which markedly reduced clonogenicity, migration, and invasion. (E) Representative xenograft tumor images. (F) Tumor growth curves. (G) Body weight changes of mice. (H) Ki67 immunohistochemistry staining. (I) Histopathological analysis of major organs. n=6 per group. Scale bar=200 μm. * P<0.05; ** P<0.01; *** P<0.001 vs DMSO; # P<0.05; ## P<0.01 vs 20 nM. HNSCC – head and neck squamous cell carcinoma.