05 May 2026 : Animal Research
RAPTOR Silencing Inhibits Head and Neck Squamous Cell Carcinoma Progression via Regulation of S100A8/A9 and Urokinase Plasminogen Activator
Zhenzhen Fu BCDEF 1,2, Song Wang BCDEF 3, Haoran Zheng BCDEF 2, Huadong Wu BCD 2, Tao Li BCF 2, Lihao Wang BC 2, Hong Li BCF 2, Qiang Zhang AEG 2*DOI: 10.12659/MSM.952169
Med Sci Monit 2026; 32:e952169
Figure 7 RAPTOR knockdown inhibits HNSCC cell aggressiveness in vitro and tumor growth in vivo. (A, B) RT-qPCR validation of RPTOR mRNA knockdown and western blot confirmation of RAPTOR protein reduction in Cal27, HSC3, and HN30 cells. (C) CCK-8 assays showing reduced proliferation upon RAPTOR knockdown. (D) Colony formation assays showing decreased clonogenicity. (E) Wound-healing assays showing impaired migration. (F) Transwell assays showing reduced invasive capacity. (G) Representative xenograft tumor images. (H) Tumor growth curves over time. (I) Ki67 immunohistochemistry staining. n=6 per group. Scale bar=200 μm. * P<0.05; ** P<0.01; *** P<0.001. HNSCC – head and neck squamous cell carcinoma; RT-qPCR – reverse transcription quantitative polymerase chain reaction.