05 May 2026 : Animal Research
RAPTOR Silencing Inhibits Head and Neck Squamous Cell Carcinoma Progression via Regulation of S100A8/A9 and Urokinase Plasminogen Activator
Zhenzhen Fu BCDEF 1,2, Song Wang BCDEF 3, Haoran Zheng BCDEF 2, Huadong Wu BCD 2, Tao Li BCF 2, Lihao Wang BC 2, Hong Li BCF 2, Qiang Zhang AEG 2*DOI: 10.12659/MSM.952169
Med Sci Monit 2026; 32:e952169
Figure 9 RAPTOR knockdown and rapamycin treatment regulates S100A8/A9–PLAU expression and PI3K/AKT/mTOR–LC3 signaling in HNSCC cells. (A) RNA-seq analysis of S100A8, S100A9, and PLAU expression in shNC vs shRPTOR Cal27 cells. (B) RT-qPCR validation of altered S100A8, S100A9, and PLAU expression after RAPTOR knockdown. (C) Western blot analysis of S100A8/9 and uPA protein levels after RAPTOR knockdown. (D) Western blot analysis of PI3K/AKT/mTOR signaling and LC3 expression after RAPTOR knockdown. (E) Western blot showing altered S100A8 and uPA protein levels after rapamycin treatment. (F) Western blot showing effects of rapamycin on PI3K/AKT/mTOR signaling and LC3 expression after RAPTOR knockdown. * P<0.05; ** P<0.01; *** P<0.001. HNSCC – head and neck squamous cell carcinoma; RT-qPCR – reverse transcription quantitative polymerase chain reaction.