10 September 2020: Review Articles
The Role of Epigallocatechin-3-Gallate in Autophagy and Endoplasmic Reticulum Stress (ERS)-Induced Apoptosis of Human DiseasesShuangshuang Zhang1ABCDE, Mengke Cao2BCDE, Fang Fang3AEFG*
Med Sci Monit 2020; 26:e924558
ABSTRACT: Tea containing abundant catechins is a popular non-alcoholic beverage worldwide. Epigallocatechin-3-gallate (EGCG) is the predominately active substance in catechins, exhibiting a wide range of functional properties including cancer suppression, neuroprotective, metabolic regulation, cardiovascular protection, stress adjustment, and antioxidant in various diseases. Autophagy, a basic cell function, participates in various physiological processes which include clearing away abnormally folded proteins and damaged organelles, and regulating growth. EGCG not only regulates autophagy via increasing Beclin-1 expression and reactive oxygen species generation, but also causing LC3 transition and decreasing p62 expression. EGCG-induced autophagy is involved in the occurrence and development of many human diseases, including cancer, neurological diseases, diabetes, cardiovascular diseases, and injury. Apoptosis is a common cell function in biology and is induced by endoplasmic reticulum stress (ERS) as a cellular stress response which is caused by various internal and external factors. ERS-induced apoptosis of EGCG influences cell survival and death in various diseases via regulating IRE1, ATF6, and PERK signaling pathways, and activating GRP78 and caspase proteins. The present manuscript reviews that the effect of EGCG in autophagy and ERS-induced apoptosis of human diseases.
Keywords: Endoplasmic reticulum stress, Cardiovascular Diseases, Catechin, Diabetes Mellitus, Endoplasmic Reticulum Chaperone BiP, Neoplasms, Nervous System Diseases
Tea is a popular non-alcoholic beverage and the second most consumed drink after water worldwide [1–3]. Depending on the antioxidant levels and fermentation degree, tea is divided into oolong, black, and green teas [2,4]. Currently, people drink tea in their daily life and the average consumption of tea is about 120 mL per day per person worldwide [5–7]. Green tea as one of the most popular and favorite teas, contains a large amount of catechins. Catechins are the main biologically active components in green tea leaves [8,9]. The catechin group includes major phenolic flavonoids, which are epicatechin, epicatechin-3-gallate, epigallocatechin, and epigallocatechin-3-gallate (EGCG). EGCG has the highest biological activity and is the most common component in total catechins [10,11]. Thus, EGCG is a common and important catechin that has been widely studied in the research of green tea [12,13].
EGCG has the highest concentration in green tea. There are 2 aromatic structures, including 3 carbon bridge structures and a hydroxyl group, in the molecule of EGCG [1,14]. EGCG has health benefits including anti-tumor , anti-inflammatory , anti-diabetes , anti-myocardial infarction , anti-cardiac hypertrophy , anti-atherosclerosis , and antioxidant  owing to its abundant phenolic hydroxyl groups. These effects are mainly related to low-density lipoprotein (LDL) cholesterol inhibition, NF-κB inhibition, MPO activity inhibition, decreased levels of glucose and glycated hemoglobin in plasma, decreased inflammatory markers, and reduced reactive oxygen species (ROS) generation .
The present manuscript reviews the effect of EGCG in autophagy and endoplasmic reticulum stress (ERS)-induced apoptosis of human diseases narratively. The references are searched for EGCG, autophagy, ERS, apoptosis in PubMed database, and those are selected from 2000–2020.
The Role of EGCG in Autophagy
Autophagy is an important cellular mechanism including cell degradation and recovery, and is highly conserved in all eukaryotes . Autophagy participates in normal physiological processes, which includes clearing away abnormally folded proteins and damaged organelles, and regulating growth . Autophagy includes microautophagy, chaperone-mediated autophagy (CMA), and macroautophagy which function to transport components to lysosomes for degradation and recycling . Microautophagy is directly involved in the phagocytosing and digesting tiny components by lysosomes [26,27], while CMA is regulated and controlled by physiological pressure [28,29]. CMA transfers the cargo into lysosome via the lysosomal membranes . In contrast to microautophagy and CMA, macroautophagy captures cellular components with autophagosomes fusing with lysosome to transport them into the cavity for degradation [31,32]. Macroautophagy is the primary and universal process that cells use to clear away damaged components. Thus, the term macroautophagy is used to represent autophagy in this manuscript.
The Role of EGCG in Autophagy and Human Diseases
In the process of tumor progression, autophagy has been found to be downregulated. Autophagy plays a role as a barrier in which a normal cell transforms into a cancerous cell since tumor cells can promote high expression of anti-autophagy genes. EGCG-induced autophagy participates in the development process of various human diseases, including cancer, neurological diseases, diabetes, cardiovascular diseases, injury, and infection. During cancer treatment, EGCG-induced autophagy not only directly affects tumor cells, but also enhances the effect of inhibiting tumor development of targeted drugs, chemotherapy, and combination therapies .
EGCG directly kills tumor cells via regulating autophagy with reactive oxygen species (ROS) and light chain 3 (LC3) transition [34,35] in many cancers, including primary effusion lymphoma, breast cancer, oral cancer, mesothelioma, and glioblastoma. In primary effusion lymphoma, EGCG significantly inhibited the growth of BABL-1 and BC-1. EGCG induced autophagy to improve cell death through LC3 transition with increasing Beclin-1 expression and formation of acidic vesicular organelles, and through enhancing ROS generation in primary effusion lymphoma cells . EGCG inhibited cell proliferation since EGCG induced autophagy by enhancing Beclin-1, ATG5, and LC3B and promoted mitochondrial depolarization in breast cancer cells. In vivo, 5, 10, and 20 mg kg−1 EGCG reduced the weight of breast cancer by 20%, 31%, and 34% and only 20 mg kg−1 EGCG significantly decreased glucose, lactic acid, and vascular endothelial growth factor (VEGF) levels . The accumulations of a series of epigenetic and genetic alterations resulted in uncontrolled proliferation division in oral squamous cells. The time-dependent maximal inhibitory concentration of EGCG is 52.3 uM in the SSC-4 cells. EGCG treatment (20 uM) inhibited the proliferation through activating autophagy via upregulating ZEB1, WNT11, IGF1R, FAS, BAK, and BAD genes and inhibiting TP53, MYC, and CASP8 genes in SSC-4 human oral squamous cells [37,38]. The main ROS coming from mitochondria played a crucial role in apoptosis and autophagy. EGCG induced autophagy by increasing the LC3-II expression levels and induced apoptosis via inducing ROS in mesothelioma cell lines, but low concentrations failed to induce cell death [35,39]. However, different concentrations of EGCG have different effects on tumor cells. In primary glioblastoma cell cultures, strong autophagy induction and apoptosis induction was observed after 500 μM EGCG, but the signs of cell death of glioblastoma cells during the observation period of 6 days were not detected under 100 nM EGCG. The data showed that EGCG with a low dose might have chemopreventive effects, but without direct cytotoxicity .
Autophagy is utilized by cancer cells to protect themselves from risk factors in order to improve their survival in targeted therapy, chemotherapy and combination therapies . In targeted therapy, EGCG-induced autophagy enhanced the sensitivity of tumor cells to target drugs. Recent studies found that drug resistance is triggered by ROS and reduced by antioxidants in lung cancer cells with gefitinib treatment [42,43]. Drug resistance of gefitinib is related to high LC3 expression and the autophagosomes markers in autophagy . EGCG overcame gefitinib resistance by inhibiting gefitinib-induced autophagy with the decreased expressions of ATG5 and LC3-II/I and the increased p62 expression in A549 cells, and EGCG suppressed tumor growth and increased the survival time in A549 xenograft mouse model . P53 is an important gene which participates in autophagy and apoptosis to promote cell survival. Dual therapy with EGCG and P53 siRNA led to activating pro-apoptotic genes and inhibiting pro-autophagy genes in the Hs578T cell model of TNBC, which suggested that this dual therapy could promote treatment effect [46,47]. The study showed that tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced apoptosis in a variety of tumor cells by activating apoptosis markers . TRAIL-induced apoptosis was inhibited by EGCG via manipulating autophagy flux and subsequently reducing the number of death receptors in TRAIL-sensitive HCT116 cells .
In chemotherapy, cisplatin as one of the most commonly used chemotherapy drugs for oral squamous cell carcinoma and colorectal cancer, and has drug resistance and serious side effects after treatment. EGCG enhances autophagy and apoptosis with activating AGTs, Beclin-1 and LC3B related pathway, and inhibiting AKT/STAT3 pathway in CAR cells [50–52]. EGCG enhances autophagy effect, which is induced by cisplatin and oxaliplatin with increasing autophagosomes, acidic vesicular organelles, and LC3-II protein in colorectal cancer cells . Clinical effect of doxorubicin (DOX), a significant chemotherapeutic drug for several tumors, is seriously limited because increasing the dose can cause severe cardiotoxicity and lowering the dose impairs treatment effect with drug resistance . In osteosarcoma, EGCG not only could reduce autophagy by decreasing SOX2OT variant 7 to enhance the growth inhibition of DOX, but also could reduce partially Notch3/DLL3 to reduce drug-resistance and the stemness of tumor cells . These results indicate that EGCG may be beneficial in cancer treatment via downregulating death markers and activating autophagy markers. However, autophagy inhibition also contributes to killing tumor cells. In the treatment of hepatocellular carcinoma, EGCG and DOX treatment produce a synergistic effect with increasing cell death by 40 to 60%, increasing apoptosis by 45%, downregulating DOX-induced autophagy and inhibiting the expression of autophagic hallmark microtubule-associated protein LC3 compared with DOX alone . In addition, photothermal-chemotherapy also ablates tumor size and improves the anticancer effect through autophagy flux and inducing the formation of autophagosomes, which is induced by the duo of DOX and EGCG in HeLa tumor models .
In combination therapies, low-intensity pulsed electric field (PEF) can improve EGCG to affect tumor cells; ultrasound (US) with tumor cells is the application of physical stimulation in cancer therapy. EGCG combined with low-energy US and low-intensity PEF treatment might cause 20% change in the survival of human pancreatic tumor cells after 72 hours. In addition, 20 μM EGCG increased intracellular ROS levels and LC3-II, and inhibited p-Akt in PANC-1 cells. And 100 μM EGCG increased LC3-II, activated caspase-3 and PARP, and reduced p-Akt in HepG2 cells to result in tumor cell death [57–59].
Neurological diseases, including Alzheimer’s disease (AD) and Parkinson’s disease (PD), produce a huge social problem in the world. Both AD and PD are very popular neurodegenerative diseases worldwide [60,61]. Neurodegenerative diseases are caused by various factors, such as the accumulation of α-synuclein in PD and β-amyloid protein in AD with increased pro-apoptotic proteins, inflammation, and oxidative stress. EGCG has been utilized as the drug for neurodegenerative diseases with extensive biological and pharmacological activities [62,63].
In neurons, autophagy can protect neuronal cells from stress to improve cell survival . EGCG protected neuronal cells against human viruses by inhibiting cytochrome c and Bax translocations, and reducing autophagy with increased LC3-II expression and decreased p62 expression . In addition, EGCG restored autophagy in the mTOR/p70S6K pathway to weaken memory and learning disorders induced by CUMS . EGCG provided the neuroprotective effect in subarachnoid hemorrhage (SAH). EGCG induced normal autophagic flux, regulated Beclin-1 and Atg5 and promptly eliminated damaged mitochondria after SAH. Finally, EGCG increased the neurological scores through inhibiting cell death . Another study found that under EGCG treatment, [Ca2+]m and [Ca2+]i expressions were reduced and oxyhemoglobin-induced mitochondrial dysfunction lessened. Finally, EGCG could rescue autophagy via regulating mRNA expressions of Becn-1, LC3B, and Atg5 after SAH. Therefore, EGCG can protect neuronal cells from or attenuate external damage through the autophagy pathway .
DIABETES AND ITS COMPLICATIONS:
Diabetes is a common metabolic disease characterized by hyperglycemia caused by insulin damage. Clinically, the main and common symptoms are polyuria, blurred vision, and weight loss. Persistent hyperglycemia leads to autonomic dysfunction, foot ulcers, renal failure, and blindness. Flavonoids are beneficial through improving the secretion of insulin, regulating glucose metabolism of hepatocytes, inhibiting the apoptosis of pancreatic β-cells, attenuating oxidative stress, inflammation and insulin resistance, and improving glucose uptake in muscle cells. And EGCG is a member of flavonoids family [69,70].
In GK (Goto-Kakizaki) rats, EGCG enhanced glucose metabolism, inhibited mitochondrial loss and dysfunction, decreased oxidative stress, and reduced autophagy by downregulating the JNK-p53 and ROS-ERK pathways in muscle cells . EGCG-induced autophagy also reduced retinal damage and relieved myocardial mitochondrial dysfunction in chronic complications of diabetes. EGCG protected Müller cells under high glucose from apoptosis through activating autophagy and restoring degradation. EGCG decreased retinal damage and inhibited the reactive gliosis of Müller cells in diabetic retinopathy models . FoxO1 plays as an important transcription factor in insulin signaling. In H9c2 cardiomyoblasts, FoxO1 induced autophagy through the ROS pathway to regulate glucose metabolism, which is inhibited by EGCG . EGCG improved mitochondrial function by increasing autophagy and upstream FoxO factors in the myocardium of diabetic models . These results showed that EGCG would be a potential drug for regulating glucose metabolism and myocardial damage involving diabetes.
Ischemic heart disease is a common cardiovascular disease with a high mortality rate. Reperfusion after myocardial ischemia can easily cause injury. EGCG pretreatment reduced autophagy induced by ischemia/reperfusion (I/R), increased cell number, and decreased myocardial infarction area. EGCG protected I/R via inhibiting autophagy through activating PI3K/Akt signals, decreasing Beclin-1, and improving MiR-384 in H9c2 cell lines . Another study found that EGCG post-treatment significantly reduced CK-MB levels and LDH release, decreased myocardial infarct area, reduced apoptosis number, and retained partial heart function in I/R injury models. In addition, EGCG post-treatment decreased I/R injury by rescuing autophagy and reducing apoptosis with reduced cleaved caspase-3, p62, Atg5, Beclin-1, and the ratio of LC3-II/LC3-I, upregulation of PI3K, cathepsin D, endothelial nitric oxide synthase, and Akt . Recent studies have shown autophagy is related to lipid metabolism, which is induced by cholesterol efflux and lipolysis in foam cells. Thence, activating autophagy promoted cholesterol outflow to hinder the formation of advanced atherosclerotic plaques in foam cells. Combined EGCG and oligomeric proanthocyanidins treatment not only activated autophagy, but also stimulated cholesterol efflux via regulating class III PI3K/Beclin-1, implying that is a potential therapeutic method for atherosclerosis .
INJURY AND BACTERIAL INFECTION:
The liver, an important metabolic organ, is closely related to autophagy. EGCG increased autophagy through promoting lysosomal acidification and improving the formation of autophagosomes in the liver. EGCG alleviated liver injury via regulating apoptosis and autophagy by inhibiting IL-6/JAKs/STAT3/BNIP3 in ConA-induced hepatitis models [78,79]. EGCG promoted cell survival via upregulating autophagy and shifting the balance of mTOR-AMPK pathways . EGCG treatment inhibited UVB-induced autophagy through reducing autophagosomes and LC3-II, and activating mTOR signals in the age-related macular degeneration. Furthermore, EGCG reduced the toxic effects of UVB on retinal pigment epithelial cells in an autophagic pathway .
In bacterial infections, EGCG limited Burkholderia cenocepacia metabolism by enhancing autophagy and inhibiting spread in cystic fibrosis, and promoted cystic fibrosis transmembrane conductance regulator (CFTR) expression . Combined EGCG and 5-AZA treatment could inhibit Legionella infection by rescuing the gene expression of autophagy in infected macrophages .
THE ROLE OF EGCG IN ENDOPLASMIC RETICULUM STRESS (ERS)-INDUCED APOPTOSIS:
Endoplasmic reticulum (ER) is a crucial organelle which can synthesize, fold, and secret various proteins in eukaryotic cells. About 30% of cellular proteins are synthesized, folded, processed, and modified to form active functional proteins, including most secreted proteins, membrane-bound proteins, and integrated membrane proteins. The new synthesized proteins encapsulated in vesicles leaves the ER and is transported to Golgi apparatus, then is directed to the inner membrane system or secreted outside the cell. Also, ER participates in some cellular functions, including calcium ion storage, gluconeogenesis, lipid and cholesterol synthesis, and formation of autophagic vesicles. When receiving endogenous or exogenous stimuli including lack of molecular chaperone or cellular energy or Ca2+, disulfide reduction, protein mutations and redox homeostasis, the function of folding protein in ER is disordered. A large number of misfolded or unfolded proteins accumulate in the cavity and cause a series of subsequent reactions called endoplasmic reticulum stress (ERS) [84–86].
ERS-INDUCED APOPTOSIS PATHWAY:
ERS is one of common stress responses, which induced apoptosis and interferes with cellular homeostasis. Upon ERS, cells will activate many adaptive functions to respond to changes in protein-folding, called unfolded protein response (UPR). UPR protects cells from stress by improving the ability of synthesizing new proteins and improving protein degradation through autophagy. When ERS is continuous and robust, the amount of the protein in the ER exceeds greatly its fold ability. This pathway clears away damaged cells through apoptosis, suggesting the mechanism controlling cell survival might depend on the intensity and duration of stress stimulation. UPR is mainly activated by 3 signaling pathways: protein kinase RNA like ER kinase (PERK), activating transcription factor-6 (ATF6), and inositol requiring protein 1 (IRE1) [84,86].
The accumulation of unfolded proteins stimulates autophosphorylation and oligomerization of IRE1α in the ER. IRE1α, TRAF2, and ASK1 form a complex to activate JNK which promotes apoptosis or autophagy . After being transferred from ER to the Golgi apparatus, ATF6 is cleaved by Site-1 and Site-2 proteases. The N-terminal cytosolic domain of cleaved ATF6 combined with ERS and cAMP response elements, and is transferred into the nucleus to activate target genes such as X-box binding protein 1 (XBP1), C/EBP homologous protein (CHOP), immunoglobulin heavy chain binding protein (BIP), and 78 kDa glucose regulated protein (GRP78). ATF6 directly regulates apoptosis and autophagy through CHOP and XBP-1 . The function of PERK is that attenuates the translation of mRNA under ERS and prevented synthesized proteins from flowing into the already stressed ER region. The activation of PERK inhibits protein translation through eIF2α phosphorylation and allows the specialized translation of transcripts including the vital sensor ATF4. ATF4 could promote apoptosis by degrading apoptosis protein inhibitors and autophagy by regulating ATG genes under prolonged ERS [84,89]. In addition, ER molecular chaperones including GRP78, BIP, GRP94, and PDI, participate in folding, assembling, and transporting of secreted and membrane proteins. See Table 1.
The Role of EGCG in ERS-Induced Apoptosis and Human Diseases
Apoptosis is a common cell function in biology and is induced by ERS as a cellular stress response which is caused by various internal and external factors. EGCG directly inhibits tumor cell growth via ERS-induced apoptosis in different cancers. In colorectal cancer cells, EGCG induced ERS in HT-29 cells by upregulating BiP, PERK, phosphorylation eIF2α, ATF4, and IRE1α. And apoptosis was induced by increased the activity of caspase-3/7 after EGCG treatment [90,91]. EGCG efficiently inhibited glucosidase II, which participates in quality control and glycoprotein processing in the ER of rat liver microsomes. EGCG interfered with protein processing owing to inhibiting glucosidase II in liver microsomes of hepatoma cells, and ERS induced incomplete UPR with main pro-apoptotic components, including increased eIF2α phosphorylation, cleavage of procaspase-12, induction of CHOP/GADD153, and depletion of ER calcium [92,93]. GRP78 can trigger UPR via activating XBP1 and CHOP in the endoplasmic reticulum to restore cell homeostasis. MMe cells with EGCG treatment improved GRP78 expression in the endoplasmic reticulum, and induced EDEM, CHOP, XBP1, and ATF4 expressions, and increased the activity of caspase-3 and caspase-8. GRP78 accumulation converted UPR of MMe cells into pro-apoptotic ERS [94,95]. Furthermore EGCG only induced ERS related apoptosis in tumor cells and was not effective for normal cells. EGCG promoted ROS production and induced apoptosis via p38 MAPK phosphorylation, caspase-8 activation, and proteolytic cleavage of Bid and JNK pathway activation in glioblastoma cells. However, EGCG did not promote apoptosis in astrocytes .
EGCG not only has a direct effect on tumors through ERS-induced apoptosis, but also promotes the sensitivity of tumor cells to therapeutic drugs or methods. siRNA downregulation of GRP78 and EGCG treatment improved apoptosis induced by celecoxib, thereby activating CHOP, caspase-4, GRP78, and IRE-1α in urothelial carcinoma cells . During chemotherapy, EGCG enhanced chrysin-induced apoptosis by overcoming GRP78 expression and enhancing caspase-7 and poly polymerase cleavage in human hepatoma cells . EGCG alone wasn’t beneficial for survival, but significantly promoted the current treatment effect of temozolomide by reducing GRP78 expression and increasing CHOP expression. And life span extension was significantly higher under combination therapy compared with temozolomide alone . EGCG enhanced the activation of ERS-induced apoptosis markers including PARP cleavage, caspase-7 and JNK phosphorylation in breast cancer with vinblastine and Taxol treatment. Inhibition of caspase-7 and JNK eliminated the sensitivity to vinblastine and Taxol in breast cancer with EGCG treatment .
However, another research reported that EGCG can antagonize chemotherapy drugs to prevent tumor cell apoptosis. EGCG effectively prevented tumor cell death mediated by BZM in glioblastoma and multiple myeloma. EGCG could only significantly antagonize the function of boronic acid proteasome inhibitors, but not the function of non-boronic acid proteasome inhibitors. Since EGCG directly reacted with BZM to block the effect of BZM, BZM could not trigger tumor cell death through activation of ERS and caspase-7. These results indicated that drinking tea would be banned during BZM therapy owing to antagonistic effect of EGCG on BZM [101,102].
Previous studies have found that EGCG protects central nervous system from diseases with its anti-inflammatory and antioxidant properties. Recent studies showed that EGCG reduced ERS-induced apoptosis by downregulating cleaved caspase-3 and caspase-12, CHOP, and GRP78 in a dose-dependent manner in SH-SY5Y Cells. Simultaneously EGCG inhibited neuronal apoptosis with reducing ER abnormal ultrastructural swelling and downregulating ERS-associated proteins in APP/PS1 transgenic mice. In conclusion, EGCG decreased the neurotoxicity via decreasing ERS related apoptosis in the AD [103,104]. In transient focal cerebral ischemia rat models, EGCG treatment after ischemia inhibited ERS with decreasing the expression of glucose-regulated caspase-12, CHOP, and GRP78, and improved the neurological status via inhibiting TRPC6 proteolysis and activating CREB in the MEK/ERK pathway . In familial amyloidotic polyneuropathy mice models, EGCG inhibited 50% deposition of transthyretin toxic that aggregates along peripheral nervous system and gastrointestinal tract system. In addition, EGCG significantly reduced the expression of the markers related non-fibrillar transthyretin deposition, including BiP, the phosphorylated eIF2a, protein oxidation marker-3-nitrotyrosine and death receptor Fas .
DIABETES AND ITS COMPLICATIONS:
The β cells of Langerhans islets absorb glucose by GLUT2, which results in the secretion of insulin to maintain glucose homeostasis. EGCG promoted the secretion of insulin and glucose tolerance, reduced the number of Langerhans pathological islets and ERS markers of the islet, increased area and number of islets, and increased the pancreatic endocrine area of db/db mice [107,108]. A-type-EGCG-dimer is beneficial for the body and affect glucose metabolism in the liver. A-type EGCG dimer prevented insulin resistance and hyperglycemia by inhibiting ERS-induced apoptosis, decreasing the levels of G6Pase and PEPCK, and the activations of ATF4, p-JNK, p-IRE1, and p-PERK in rat liver . Podocytes participate in maintaining the integrity of the glomerular filtration barrier and preventing the production of proteinuria. Podocyte injury can affect the development and prognosis of diabetic nephropathy. EGCG attenuated apoptosis of glucose-induced podocyte through inhibiting ERS with attenuating the expressions of caspase-12, p-PERK, and GRP78 in mouse podocytes . It has been found that activating nuclear factor erythroid 2 related factor 2 (NRF2) can attenuate diabetic testicular damage in rodents. EGCG reduced apoptosis of testicular cell by ERS, oxidative damage, and inflammation, and activated the expression of NRF2 in diabetic mice .
Endothelial dysfunction is a common cause of cardiovascular diseases, which is affected by many factors including oxidative stress, renin-angiotensin system, oxidized low density lipoprotein, and homocysteine. ERS related NOD-like receptor pyrin domain containing-3 (NLRP3) and thioredoxin-interacting protein (TXNIP) signals are important factors in the endothelial dysfunction and induce inflammation and cell death by producing IL-1β. EGCG is a member of flavonoids groups and has beneficial effects on cardiovascular diseases. One study showed that EGCG inhibited ROS levels and the activation of NLRP3 and TXNIP inflammasome, resulting in decreased IL-1β expression. Simultaneously EGCG reduced cell apoptosis by inhibiting the activity of caspase-3 and restoring mitochondrial membrane potential. EGCG inhibited the activation of NLRP3 and TXNIP inflammasome by regulating the activity of AMPK to protecting endothelial cells from inflammatory and apoptosis [112,113].
ADVERSE REACTIONS AND INJURY:
Cisplatin (CP) is widely used in the chemotherapy for various cancers and is limited high-dose treatment due to its serious adverse reactions, especially nephrotoxicity. CP causes acute renal injury via renal tubular dysfunction in many patients. In CP-induced nephrotoxicity mice, EGCG reduced immunohistochemical damage and biochemical factors, and decreased the expression of phosphorylated ERK, GRP78, and caspase-12. EGCG reduced renal apoptosis by inhibiting ERS [114–116]. Partial bladder outlet obstruction (pBOO) caused by many etiologies, including benign prostatic hyperplasia, cystocele, posterior urethral valves, bladder stones, and urethral stricture, is a common disease of the urinary tract. After the female Sprague-Dawley rats received EGCG treatment, bladder injury and dysfunction was significantly promoted by inhibiting inflammation and ERS-related apoptosis with increased the expression of caspase-12, CHOP, and cyclooxygenase-2 at 48 hours and 30 days [117,118]. ERS-induced apoptosis closely correlated with the progression of age-related macular degeneration. ERS caused the accumulation of misfolded proteins and activated UPR to promote cell survival. EGCG inhibited ERS-mediated apoptosis via downregulating cleaved caspase-12, cleaved caspase-3, cleaved PARP, IRE1α, ERO1α, PERK, CHOP, GRP78, and phosphorylation at ser9 of GSK3β, and upregulating the expression of phosphorylation ser380 of PTEN and ser473 of AKT in retinal pigment epithelial cells [119,120]. See Table 2.
Autophagy and apoptosis closely correlated with the progression of human diseases. As EGCG is associated with apoptosis and autophagy, EGCG exhibits a wide range of functional properties, including cancer prevention, neuroprotection, anti-cardiovascular effect, and anti-diabetic effect (Figure 1). EGCG not only regulates autophagy via increasing Beclin-1 expression and ROS generation, but also causing LC3 transition and decreasing p62 expression. ERS-induced apoptosis of EGCG influences cell survival and death in various diseases via regulating IRE1, ATF6, and PERK signaling pathways, and activating GRP78 and caspase proteins. EGCG-induced autophagy and ERS-induced apoptosis of EGCG is involved in human diseases, including cancer, neurological diseases, diabetes, cardiovascular diseases, and injury.
As natural compounds can interact with the complex network of interlinked physiological processes, these findings of EGCG obtained from a number of experimental models and cell lines cannot be directly inferred into humans. Humans and other organisms have conservative mechanisms, and these mechanisms were inherited in the course of biological evolution. But the models and cell lines of EGCG are not perfectly matched owing to human complex physiology [3,121]. In addition, EGCG metabolism in the intestine and the circulatory systems of these models were unique in different studies . For example, there are different effects of different concentration EGCG in different model organisms for cancer therapy in the manuscript. Future studies should focus on humans to determine whether the same benefits in the models can be replicated. Clinical trials will be conducted to determine the optimal dosage of EGCG to achieve the maximum health benefits of humans, which will be the novel and potential leap in basic and clinical research. Researchers need to make more efforts to convert large amounts of preclinical data into effective human treatment methods.
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