04 May 2007
Significant discrepancy between real-time PCR identification and hospital identification of C. albicans from Lebanese patientsSuha Yazbek, Ghyda Barada, Raida Basma, Jad Mahfouz, Roy A. Khalaf
Med Sci Monit 2007; 13(5): MT7-12 :: ID: 484614
Background: The fungus C. albicans is among the leading agents causing death in immunocompromised patients. Most hospitals rely on conventional morphological techniques such as the germ-tube assay
and the API system for correct identifi cation. This technique is subjective and hence error prone. Recently, more and more molecular techniques for correct identifi cation have been developed. The latest is the LightCycler real-time PCR technique coupled with melting curve analysis.
Material/Methods: One hundred hospital isolates presumed to be C. albicans from four major Lebanese hospitals were tested using the real-time PCR technique. The results were compared with a germ-tube test.
Furthermore, all real-time PCR-positive samples were replica plated on growth media at 30°C and 45°C to differentiate between C. albicans and C. dubliniensis. Finally, all PCR-negative samples were identifi ed using the API 20C AUX yeast identifi cation system.
Results: Twenty-four hospital isolates were non-albicans by PCR (ppC. albicans-negative isolates were misidentifi ed by the API 20C AUX. None of the C. albicans real-time PCR-positive samples failed to grow at 45°C, the
dubliniensis non-permissive temperature.
Conclusions: Considering the impact of false identifi cation on the general public health through the use of wrong antifungal drug treatment and the emergence of novel drug-resistant strains, hospitals should update their classifi cation methods using molecular techniques.
Keywords: Candidiasis - drug therapy, Candida albicans - genetics, Antifungal Agents - therapeutic use, Drug Resistance, Fungal, Hospitals, Lebanon, Mycological Typing Techniques, Polymerase Chain Reaction - methods
01 March 2023 : EditorialEditorial: Global Surveillance of Highly Pathogenic Avian Influenza Viruses in Poultry, Wild Birds, and Mammals to Prevent a Human Influenza Pandemic
Med Sci Monit 2023; 29:e939968
09 February 2023 : Clinical ResearchEvaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...
Med Sci Monit 2023; 29:e938892
19 January 2023 : Clinical ResearchDetermining the Impact of the COVID-19 Pandemic on Dental Care in the Serbian Adult Population: An Online Q...
Med Sci Monit 2023; 29:e939238
27 December 2022 : Clinical ResearchEffect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...
Med Sci Monit 2022; 28:e938926
10 December 2022 : Clinical ResearchRetrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...
Med Sci Monit 2022; 28:e938647
27 Mar 2023 : Database AnalysisRelationship Between Physical Activity and Adult Asthma Control Using NHANES 2011-2020 Data
Med Sci Monit In Press; DOI: 10.12659/MSM.939350
27 Mar 2023 : Clinical ResearchEvaluation of the One-Hour ¹³C-Propionate Breath Test in 49 Patients from a Single Center in Japan to Detec...
Med Sci Monit In Press; DOI: 10.12659/MSM.940238
24 Mar 2023 : Database AnalysisCauses of Death Following Gastric Cancer Diagnosis: A Population-Based Analysis
Med Sci Monit In Press; DOI: 10.12659/MSM.939848
22 Mar 2023 : Special reportOne Year On: Poland’s Public Health Initiatives and National Response to Millions of Refugees from Ukraine
Med Sci Monit In Press; DOI: 10.12659/MSM.940223
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : EditorialEditorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...
Med Sci Monit 2022; 28:e935952