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01 October 2011

The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material

Jacek SzeligaABCDEF, Ewa KlodzinskaABDEF, Marek JackowskiADEG, Boguslaw BuszewskiADEG

DOI: 10.12659/MSM.881967

Med Sci Monit 2011; 17(10): MT91-96


Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.
Material/Methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.
Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).
Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

Keywords: Surgical Wound Infection - microbiology, Sensitivity and Specificity, Escherichia coli Infections - diagnosis, Electrophoresis, Capillary - methods, Edetic Acid, Boric Acids, Tromethamine

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750