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22 December 2011

18alpha-Glycyrrhizin induces apoptosis and suppresses activation of rat hepatic stellate cells

Ying QuBCE, Wei-Hua ChenB, Lei ZongD, Ming-Yi XuF, Lun-Gen LuAG

DOI: 10.12659/MSM.882196

Med Sci Monit 2012; 18(1): BR24-32

Abstract

Background: To investigate the potential mechanisms underlying the protective effects of 18alpha Glycyrrhizin (GL) on rat hepatic stellate cells (HSCs) and hepatocytes in vivo and in vitro.
Material/Methods: Sprague-Dawley (SD) rats were randomly divided into 5 groups: normal control group, liver fibrosis group, high-dose 18alpha GL group (25 mg/ kg/d), intermediate-dose 18alpha GL group (12.5 mg/kg/d) and low-dose 18alpha GL group (6.25 mg/ kg/d). The rat liver fibrosis model was induced by carbon tetrachloride (CCl4). The expressions of alpha-smooth muscle actin (alphaSMA) and NF-kappaB were determined by real-time PCR and immunohistochemistry.
Results: 18alphaGL dose-dependently inhibited the CCl4-induced liver fibrosis. There were significant differences in the mRNA and protein expressions of alphaSMA between the fibrosis group and 18alpha-GL treatment groups, suggesting that 18alpha GL can suppress the proliferation and activation of HSCs. Few HSCs were apoptotic in the portal area and fibrous septum in the liver fibrosis group. However, the double-color staining of a-SMA and TUNEL showed that 18alpha-GL treatment groups increased HSC apoptosis. NF-kappaB was mainly found in the nucleus in the fibrosis group, while cytoplasmic expression of NF-kappaB was noted in the 18alphaGL groups. In the in vitro experiments, 18alpha GL promoted the proliferation of hepatocytes, but inhibited that of HSCs. HSCs were arrested in the G2/M phase following 18alpha GL treatment and were largely apoptotic.
Conclusions: 18alpha-GL can suppress the activation of HSCs and induce the apoptosis of HSCs by blocking the translocation of NF-kappaB into the nucleus, which plays an important role in the protective effect of 18alpha-GL on liver fibrosis.

Keywords: Liver Cirrhosis - drug therapy, In Situ Nick-End Labeling, Hepatic Stellate Cells - drug effects, Glycyrrhizic Acid - therapeutic use, Dose-Response Relationship, Drug, Cell Proliferation - drug effects, Carbon Tetrachloride - toxicity, Real-Time Polymerase Chain Reaction

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750