Abstract

Background: To identify meningioma-specific proteins, cerebrospinal fluid (CSF) from 4 patients with a meningioma and 4 patients with a non-brain tumorous lesion were analyzed.
Material/Methods: Two-dimensional electrophoresis and electrospray quadrupole time-of-flight tandem mass spectrometry analyses revealed 10 unique spots, containing 11 independent proteins (spot #2 and #4 each contained 2 proteins and spot #3 was not identified) were evident in CSF associated with human meningioma: serum albumin precursor (3 different isoforms), Apolipoprotein E (Apo E), Apolipoprotein J precursor (Apo J), Transthyretin precursor (TTR), Prostaglandin D2 synthase 21kDa (PTGDS), proapolipoprotein, Chain D hemoglobin Ypsilanti, alpha-1-antitrypsin (AAT), and beta-2-microglobulin precursor (beta2M).
Results: The contents of Apo E, Apo J and AAT were increased, while PTGDS, TTR and beta2M were decreased.
Conclusions: The results observed by 2-dimensional electrophoresis were verified by Western blot analysis. The unique proteins may represent possible candidate biomarkers of meningioma.

Keywords: Molecular Sequence Data, Neoplasm Proteins - chemistry, Molecular Sequence Annotation, Meningioma - diagnosis, Meningeal Neoplasms - diagnosis, Electrophoresis, Gel, Two-Dimensional, Blotting, Western, Amino Acid Sequence, Proteome - metabolism, Proteomics - methods, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization, Tumor Markers, Biological - cerebrospinal fluid