Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

30 April 2015 : Laboratory Research  

The Role of Nrf2 in Pathology of Pleomorphic Adenoma in Parotid Gland

Agnieszka DroździkABCDEF, Robert KowalczykB, Ewa JaworowskaB, Elżbieta UrasińskaBDE, Mateusz KurzawskiBC

DOI: 10.12659/MSM.892674

Med Sci Monit 2015; 21:1243-1248

Abstract

BACKGROUND: Pleomorphic adenoma (benign mixed tumor) is one of the most common salivary gland tumors. However, the processes involved in its carcinogenesis are not well defined. This study aimed to define the contribution of Nfr2 (nuclear factor (erythroid-derived 2)-like 2) to pleomorphic adenoma pathology. The Nrf2-controlled gene system is one of the most critical cytoprotective mechanisms, providing antioxidant responses.

MATERIAL AND METHODS: The study was carried out in pleomorphic adenoma and control parotid gland tissues, investigating gene expression of NFE2L2, as well as KEAP1 (Kelch-like ECH-associated protein 1) and NQO1 (quinone oxidoreductase), at mRNA and protein (immunohistochemistry) levels. Functional evaluation of Nrf2 system in the parotid gland was evaluated in HSY cells (human parotid gland adenocarcinoma cells).

RESULTS: Pleomorphic adenoma specimens showed cytoplasmic and nuclear Nfr2 expression in epithelial cells, as well as more variable lower Nrf2 level in mesenchymal cells. In the parotid gland, Nrf2 was expressed in cytoplasm of serous, mucous, and duct cells. Nuclear Nrf2 expression was predominantly seen in serous cells, whereas mucous and duct cells were mostly negative. Comparable mRNA levels of NFE2L2 and NQO1 genes and significantly higher expression of KEAP1 in pleomorphic adenoma were seen. HSY cell incubation with oltipraz demonstrated significant elevation of NFE2L2 after 24 and 48 hours of stimulation, whereas NQO1 was elevated, but significantly only after 24 hours, and KEAP1 expression remained unchanged.

CONCLUSIONS: Summarizing both in vitro and in vivo observations, it can be stated that Nrf2 may play a role in the pathology of pleomorphic adenoma.

Keywords: Adenoma, Pleomorphic - pathology, Epithelial Cells - pathology, Intracellular Signaling Peptides and Proteins - metabolism, NAD(P)H Dehydrogenase (Quinone) - genetics, NF-E2-Related Factor 2 - metabolism, Parotid Neoplasms - pathology, RNA, Messenger - metabolism, RNA, Neoplasm - metabolism, Tumor Cells, Cultured

Add Comment 0 Comments

Editorial

01 December 2024 : Editorial  

Editorial: The 2024 Revision of the Declaration of Helsinki and its Continued Role as a Code of Ethics to Guide Medical Research

Dinah V. Parums

DOI: 10.12659/MSM.947428

Med Sci Monit 2024; 30:e947428

0:00

In Press

Clinical Research  

Evaluating Lipiodol Efficacy in Proximal Tubal Occlusion Treatment: Single-Center Experience and Literature...

Med Sci Monit In Press; DOI: 10.12659/MSM.946266  

Review article  

Impact of Traditional Chinese Medicine Antioxidants on Oxidative Stress and Drug-Induced Liver Injury: A Re...

Med Sci Monit In Press; DOI: 10.12659/MSM.945147  

Meta-Analysis  

Reliability of Extraoral Scanners in Capturing 3D Geometry for Dental Prostheses: A Systematic Review

Med Sci Monit In Press; DOI: 10.12659/MSM.946470  

Review article  

Skin Barrier Dysfunction in Acne Vulgaris: Pathogenesis and Therapeutic Approaches

Med Sci Monit In Press; DOI: 10.12659/MSM.945336  

Most Viewed Current Articles

17 Jan 2024 : Review article   6,957,034

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research   1,969,335

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research   697,010

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

07 Jan 2022 : Meta-Analysis   263,050

Efficacy and Safety of Light Therapy as a Home Treatment for Motor and Non-Motor Symptoms of Parkinson Dise...

DOI :10.12659/MSM.935074

Med Sci Monit 2022; 28:e935074

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750