23 November 2015 : Laboratory Research
Impact of Mitochondria-Mediated Apoptosis in U251 Cell Cycle Arrest in G1 Stage and Caspase ActivationLei ZhangACD, Peng LiangBDE, Rui ZhangFG
Med Sci Monit 2015; 21:3629-3633
BACKGROUND: Most mitochondria-mediated apoptosis has some relevance to the cell cycle, but there is still a lack of investigations about U251 cell cycle in human brain glioma cells. In this study, we aimed to clarify the correlation of mitochondria-mediated apoptosis with the U251 cell cycle and its influence on apoptosis, through observing the impact of mitochondria-mediated apoptosis in U251cell specificity cycle arrest and Caspase activation.
MATERIAL AND METHODS: AnnexinV/PI and API were used to label the brain glioma cells for flow cytometry analysis of U251 cell apoptosis and cell cycle. RT-PCR and Western blot were performed to detect Caspase-3 and Caspase-9 activation.
RESULTS: Peripheral blood in stationary phase is not sensitive to apoptosis induction, but U251 cells have obvious apoptosis. Mitochondria-mediated apoptosis mainly occurs in the G1 phase of the cell cycle. Caspase-3 and Caspase-9 mRNAs and proteins expression increased significantly after the cells were treated by mitochondrial apoptosis-related gene Bax induction.
CONCLUSIONS: Mitochondria-mediated apoptosis is related to the U251 cell cycle with specific G1 stage arrest. Caspase activation occurs in the process of cell apoptosis.
Keywords: Brain Neoplasms - pathology, Apoptosis - physiology, Caspase Inhibitors - metabolism, Caspases - metabolism, Cell Proliferation - physiology, G1 Phase Cell Cycle Checkpoints - physiology, Glioma - pathology, Mitochondria - physiology
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