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15 October 2015 : Laboratory Research  

MiR-29 Induces K562 Cell Apoptosis by Down-Regulating FoxM1

Xiaofang WangDE, Hua ZhongCD, Lei WangBG, Yuqian DongBD, Ankui JiaBD, Qingjiang MoFG, Chenguang ZhangABC

DOI: 10.12659/MSM.894554

Med Sci Monit 2015; 21:3115-3120

Abstract

BACKGROUND: Leukemia seriously threatens human life and health. MicroRNAs can regulate cell growth, proliferation, and death. This article investigated the role of miR-29 on regulating leukemia cell growth, proliferation, and apoptosis.

MATERIAL AND METHODS: miR-29 and scramble miRNA were transfected to K562 cells. MTT assay, colony formation assay, caspase-3 activity detection, and flow cytometry were applied to test miR-29 effect on cell growth, proliferation, and apoptosis. Western blot was used to detect Forkhead box protein M1 (FoxM1) protein expression. After we transfected miR-29, K562 cells were transfected with FoxM1 siRNA to test cell apoptosis.

RESULTS: K562 cell growth and proliferation were inhibited after transfection with miR-29. Apoptosis phenome and caspase-3 activation were observed. FoxM1 level decreased. SiRNA FoxM1 enhanced miR-29-induced K562 cell apoptosis. FoxM1 overexpression suppressed miR-26-induced K562 cell apoptosis.

CONCLUSIONS: MiR-29 restrained K562 cell growth and proliferation. MiR-29 induced K562 cell apoptosis through down-regulating FoxM1.

Keywords: Forkhead Transcription Factors - metabolism, Gene Expression Regulation, Leukemic, K562 Cells, RNA, Messenger - metabolism

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750