22 November 2015 : Animal Research
Gene Expression and Correlation of PTEN and FABP4 in Liver, Muscle, and Adipose Tissues of Type 2 Diabetes RatsDi SuABC, Chuan-ling ZhangACE, Ying-chun GaoF, Xiao-ying LiuB, Cai-ping LiB, Jian HuangfuBC, Rui XiaoDEG
Med Sci Monit 2015; 21:3616-3621
BACKGROUND: The aim of this work was to study the FABP4 and PTEN gene expression and correlation in the liver, muscle, and adipose tissues of type 2 diabetes mellitus (T2DM) rats.
MATERIAL AND METHODS: Male Wistar rats (8 weeks old) were randomly divided into 2 groups (n=12/group): a control group fed a normal diet for 8 weeks and an experimental group fed a high-fat, high-sugar diet for 8 weeks and that received 25 mg/kg streptozotocin by intraperitoneal injection to induce T2DM. The random blood glucose, fasting blood glucose, and fasting insulin levels were measured. The expression of Pten and Fabp4 in the liver, muscle, and epididymal adipose tissues was estimated by real-time quantitative PCR. Pearson correlation coefficient analysis was used to investigate the expression correlation between Pten and Fabp4 in T2DM rats.
RESULTS: The gene expressions of Pten and Fabp4 in the liver, muscle, and adipose tissues of T2DM rats were all significantly higher than those in the control group (P<0.05). Pten was highly expressed in the muscles and Fabp4 was highly expressed in muscle and adipose tissues. Furthermore, expressions of Fabp4 and Pten in the muscle and adipose tissues of T2DM rats were positively correlated (P<0.05), but not in the liver.
CONCLUSIONS: The increased expression of PTEN and FABP4 in the adipose and muscles of T2DM rats may play an important role in the insulin resistance of T2DM. However, the mechanism by which these 2 genes function in T2DM needs further study.
Keywords: Adiposity, Adipose Tissue - metabolism, Blood Glucose - metabolism, Diabetes Mellitus, Experimental - metabolism, Diabetes Mellitus, Type 2 - metabolism, Fatty Acid-Binding Proteins - metabolism, Gene Expression Profiling, Insulin - metabolism, Insulin Resistance, Liver - metabolism, Muscles - metabolism, Obesity - metabolism, PTEN Phosphohydrolase - metabolism, Rats, Wistar
12 September 2022 : EditorialEditorial: Treatment with Dual Incretin Receptor Agonists to Maintain Normal Glucose Levels May Also Maintain Normal Weight and Control Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD)
Med Sci Monit 2022; 28:e938365
16 September 2022 : Review articleEffects of Physiotherapy on Rehabilitation and Quality of Life in Patients Hospitalized for COVID-19: A Rev...
Med Sci Monit In Press; DOI: 10.12659/MSM.938141
12 September 2022 : Clinical ResearchEffect of Vitamin D Concentration on Course of COVID-19
Med Sci Monit In Press; DOI: 10.12659/MSM.937741
29 August 2022 : Database AnalysisTranscriptome Analysis of Peripheral Blood Mononuclear Cells Response in Patients with Severe COVID-19 Reve...
Med Sci Monit 2022; 28:e937532
23 Sep 2022 : Review articleA Review of the Role of Ultrasound Radiomics and Its Application and Limitations in the Investigation of Th...
Med Sci Monit In Press; DOI: 10.12659/MSM.937738
23 Sep 2022 : Review articleReview of the Long-Term Outcomes of Guided Bone Regeneration and Autologous Bone Block Augmentation for Ver...
Med Sci Monit In Press; DOI: 10.12659/MSM.937433
22 Sep 2022 : Clinical ResearchComparison of Incremental Shuttle Walking Test, 6-Minute Walking Test, and Cardiopulmonary Exercise Stress ...
Med Sci Monit In Press; DOI: 10.12659/MSM.938140
Most Viewed Current Articles
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292