19 October 2015 : Laboratory Research
MiR-1271 Inhibits Ovarian Cancer Growth by Targeting Cyclin G1Xiaogang LiuAG, Lihong MaBC, Qinhua RaoDE, Yuhui MaoEF, Yuhong XinBC, Huiqin XuDE, Changju LiBC, Xiaoyan WangCD
Med Sci Monit 2015; 21:3152-3158
BACKGROUND: Ovarian cancer is the most lethal gynecological malignant cancer in the female genital system. The dysfunction of miRNA contributes to ovarian cancer development.
MATERIAL AND METHODS: The miR-1271 level in ovarian cancer tissues and cells was assayed by qRT-PCR. The miR-1271 expression in cells was overexpressed by miRNA-mimic transfection and reduced by miRNA-antisense-oligonucleotide (ASO) transfection. Cell proliferation was analyzed by an MTT assay. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. The protein level was assayed by Western blotting.
RESULTS: The ovarian cancer tissue and cell lines showed low levels of miR-1271. Low levels of miR-1271 in ovarian cancer tissues were correlated with a low rate of patient survival, and the overexpression of miR-1271 inhibited the proliferation of ovarian cancer cells. The 3’ UTR of cyclin G1 (CCNG1) was targeted by miR-1271.
CONCLUSIONS: Low levels of miR-1271 in ovarian cancer tissues promoted cancer cell growth. MiR-1271 may be a new predictor of prognosis in ovarian cancer. MiR-1271 exerted its role by targeting CCNG1.
Keywords: Algorithms, Cyclin G1 - metabolism, Ovarian Neoplasms - metabolism, Prognosis
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