10 August 2020: Lab/In Vitro Research
Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells
Xianhao Su ACF* , Liang Chen AD* , Shanliang Han B , Gengming Niu F , Jun Ren C , Chongwei Ke AG*DOI: 10.12659/MSM.922935
Med Sci Monit 2020; 26:e922935
Table 1 Frequency distribution of pore size of scaffolds made from silk fibroin (SF), chitosan (Cs), alginate (Alg), SF/Cs (1: 1), SF/Cs/Alg (1: 1: 0.5), SF/Cs/Alg (1: 1: 1), and SF/Cs/Alg (1: 1: 2). Image J was used to measure the cross-sectional area of pore for each group of scaffolds and the diameter of each pore was obtained by the formula: Area=πd2/4, (d – diameter; NA – not available).
| Pore-size distrbution | Groups | ||||||
|---|---|---|---|---|---|---|---|
| SF | Cs | Alg | SF/Cs (1: 1) | SF/Cs/Alg (1: 1: 0.5) | SF/Cs/Alg (1: 1: 1) | SF/Cs/Alg (1: 1: 2) | |
| NA | 13 | NA | 0 | 0 | 0 | 17 | |
| 51–100 μm (‰) | NA | 320 | NA | 13 | 10 | 0 | 85 |
| 101–200μm (‰) | NA | 540 | NA | 204 | 485 | 125 | 193 |
| 201–300 μm (‰) | NA | 120 | NA | 566 | 317 | 152 | 210 |
| 301–400 μm (‰) | NA | 7 | NA | 164 | 149 | 241 | 223 |
| 401–500 μm (‰) | NA | 0 | NA | 33 | 9 | 223 | 92 |
| 501–600 μm (‰) | NA | 0 | NA | 7 | 20 | 107 | 91 |
| 601–700 μm (‰) | NA | 0 | NA | 6 | 10 | 116 | 61 |
| >701 μm (‰) | NA | 0 | NA | 7 | 0 | 36 | 28 |
| Mean value (μm) | NA | 132 | NA | 255 | 221 | 400 | 319 |
| Standard deviation | NA | 56 | NA | 85 | 91 | 158 | 182 |
| Coefficient of variation (%) | NA | 42 | NA | 33 | 41 | 39 | 57 |