10 August 2020 : Laboratory Research
Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro
Xianhao Su1ACE, Liang Chen1AD, Shanliang Han1B, Gengming Niu1F, Jun Ren1C, Chongwei Ke1AG*DOI: 10.12659/MSM.922935
Med Sci Monit 2020; 26:e922935
Table 1 Frequency distribution of pore size of scaffolds made from silk fibroin (SF), chitosan (Cs), alginate (Alg), SF/Cs (1: 1), SF/Cs/Alg (1: 1: 0.5), SF/Cs/Alg (1: 1: 1), and SF/Cs/Alg (1: 1: 2). Image J was used to measure the cross-sectional area of pore for each group of scaffolds and the diameter of each pore was obtained by the formula: Area=πd2/4, (d – diameter; NA – not available).
| Pore-size distrbution | Groups | ||||||
|---|---|---|---|---|---|---|---|
| SF | Cs | Alg | SF/Cs (1: 1) | SF/Cs/Alg (1: 1: 0.5) | SF/Cs/Alg (1: 1: 1) | SF/Cs/Alg (1: 1: 2) | |
| <50 μm (‰) | NA | 13 | NA | 0 | 0 | 0 | 17 |
| 51–100 μm (‰) | NA | 320 | NA | 13 | 10 | 0 | 85 |
| 101–200μm (‰) | NA | 540 | NA | 204 | 485 | 125 | 193 |
| 201–300 μm (‰) | NA | 120 | NA | 566 | 317 | 152 | 210 |
| 301–400 μm (‰) | NA | 7 | NA | 164 | 149 | 241 | 223 |
| 401–500 μm (‰) | NA | 0 | NA | 33 | 9 | 223 | 92 |
| 501–600 μm (‰) | NA | 0 | NA | 7 | 20 | 107 | 91 |
| 601–700 μm (‰) | NA | 0 | NA | 6 | 10 | 116 | 61 |
| >701 μm (‰) | NA | 0 | NA | 7 | 0 | 36 | 28 |
| Mean value (μm) | NA | 132 | NA | 255 | 221 | 400 | 319 |
| Standard deviation | NA | 56 | NA | 85 | 91 | 158 | 182 |
| Coefficient of variation (%) | NA | 42 | NA | 33 | 41 | 39 | 57 |