29 June 2020 : Animal Research
Kupffer Cells Regulate Natural Killer Cells Via the NK group 2, Member D (NKG2D)/Retinoic Acid Early Inducible-1 (RAE-1) Interaction and Cytokines in a Primary Biliary Cholangitis Mouse Model
Hai-Yan Fu1EF, Wei-Min Bao2C, Cai-Xia Yang1D, Wei-Ju Lai1D, Jia-Min Xu1B, Hai-Yan Yu1B, Yi-Na Yang1B, Xu Tan1B, Ajay Kumar Gupta1F, Ying-Mei Tang1AG*DOI: 10.12659/MSM.923726
Med Sci Monit 2020; 26:e923726
Table 1 Culture system design.
| Culture system | Group | Culture program |
|---|---|---|
| KCs | PBC | Primary culture of PBC Kupffer cells |
| CON | Primary culture of CON Kupffer cells | |
| NK | PBC | Primary culture of PBC NK cells |
| CON | Primary culture of CON NK cells | |
| KCs+NK | PBC | PBC Kupffer cells+CON NK cells |
| CON | CON Kupffer cells+ PBC NK cells | |
| KCs+LPS1 | PBC | 1 mg/mL LPS+PBC Kupffer cells |
| CON | 1 mg/mL LPS+ CON Kupffer cells | |
| KCs+LPS10 | PBC | 10 mg/mL LPS+PBC Kupffer cells |
| CON | 10 mg/mL LPS+ CON Kupffer cells | |
| KCs+NK+LPS1 | PBC | 1 mg/mL LPS+PBC Kupffer cells+CON NK cells |
| CON | 1 mg/mL LPS+CON Kupffer cells+PBC NK cells | |
| KCs+NK+LPS1/B | PBC | 1 mg/mL LPS+PBC Kupffer cells+CON NK cells+antibody blocking |
| CON | 1 mg/mL LPS+CON Kupffer cells+PBC NK cells+antibody blocking | |
| KCs+NK+LPS10 | PBC | 10 mg/mL LPS+PBC Kupffer cells+CON NK cells |
| CON | 10 mg/mL LPS+CON Kupffer cells+PBC NK cells | |
| KCs+NK+LPS10/B | PBC | 10 mg/mL LPS+ PBC Kupffer cells+CON NK cells+antibody blocking |
| CON | 10 mg/mL LPS+ CON Kupffer cells+PBC NK cells+antibody blocking | |
| NK – natural killer; LPS – lipopolysaccharide; PBC – primary biliary cholangitis; CON – control. | ||