09 July 2020 : Laboratory Research
Long Non-Coding RNA (lncRNA) Small Nucleolar RNA Host Gene 15 (SNHG15) Alleviates Osteoarthritis Progression by Regulation of Extracellular Matrix Homeostasis
Yunping Chen1AB, Hongna Guo1AB, Lang Li23A, Dingsu Bao24B, Feng Gao3C, Qiang Li3D, Qi Huang3D, Xin Duan2F, Zhou Xiang2AG*DOI: 10.12659/MSM.923868
Med Sci Monit 2020; 26:e923868
Table 1 The primer information for qRT-PCR assay.
| Gene | Name | Sequence |
|---|---|---|
| KLF4 | Forward | 5′-GTCCCGGGGATTTGTAGCTC-3′ |
| Reverse | 5′-TGTAGTGCTTTCTGGCTGGG-3′ | |
| Aggrecan | Forward | 5′-CAGACCATGACAACTCGCTG-3′ |
| Reverse | 5′-GCAGCACTACCTCCTTCTCC-3′ | |
| MMP3 | Forward | 5′-TGAGGACACCAGCATGAACC-3′ |
| Reverse | 5′-ACTTCGGGATGCCAGGAAAG-3′ | |
| COL2A1 | Forward | 5′-GCAGGATGGGCAGAGGTATAA-3′ |
| Reverse | 5′-CGAGGTCAGTTGGGCAGATG-3′ | |
| ADAMTS5 | Forward | 5′-CTCGGGAGGATTTATGTG-3′ |
| Reverse | 5′-ATGCTGGTAAGGATGGAA-3′ | |
| SNHG15 | Forward | 5′-CTAGTCATCCACCGCCATCC-3′ |
| Reverse | 5′-TTTGAGACCGTCACCTCAGC-3′ | |
| β-catenin | Forward | 5′-GCGCCATTTTAAGCCTCTCG-3′ |
| Reverse | 5′-GGCCATGTCCAACTCCATCA-3′ | |
| β-actin | Forward | 5′-AGGGGCCGGACTCGTCATACT-3′ |
| Reverse | 5′-GGCGGCACCACCATGTACCCT-3′ |