01 August 2020 : Clinical Research
Effects of Occupational Stress and Circadian CLOCK Gene Polymorphism on Sleep Quality of Oil Workers in Xinjiang, China
Li Ning1BCDEF, Lingyun Shi2BCDEF, Ning Tao1BC, Rong Li1BC, Ting Jiang1BC, Jiwen Liu1ABEG*DOI: 10.12659/MSM.924202
Med Sci Monit 2020; 26:e924202
Table 2 The PCR condition of each gene.
| Gene | Locus | Cycle | Further extension |
|---|---|---|---|
| CLOCK | rs1801260 | 94°C 40 s, 54°C 30 s, 72°C 60 s | 72°C 5 min |
| 35 cycles | |||
| rs6850524 | 94°C 40 s, 54°C 60 s, 72°C 60 s | 72°C 5 min | |
| 35 cycles | |||
| Per2 | rs2304672 | 94°C 45 s, 59°C 45 s, 72°C 45 s | 72°C 7 min |
| 30 cycles | |||
| Per3 | 94°C 40 s, 58°C 30 s, 72°C 40 s | 72°C 12 min | |
| 35 cycles |