19 September 2020 : Laboratory Research
Apigenin Mitigates Intervertebral Disc Degeneration through the Amelioration of Tumor Necrosis Factor α (TNF-α) Signaling Pathway
Fan Ding1ABCDEF, Xia Li2ABCDEFG*DOI: 10.12659/MSM.924587
Med Sci Monit 2020; 26:e924587
Table 1 Primer details of the study.
| Gene | Primer | Sequence | Annealing |
|---|---|---|---|
| Asporin | F | CCTGGTAGGAGGGCTGGATT | 59 |
| R | CCTTCATGCTGTCCCGTGTA | ||
| Syndecan-4 | F | TGTTTTTGACCCTGGCCCTT | 59 |
| R | CTCGATCTCGAGAAGGCACC | ||
| TNF-α | F | AAGCTGTCTTCAGGCCAACA | 57 |
| R | CCCGTAGGGCGATTACAGTC | ||
| TNF-R1 | F | TTTTCTCCAGGCAGCAACCA | 56 |
| R | GCTAGCAGGACAGTCAAGGG | ||
| COX-2 | F | AAGGCGTTCAACTGAGCTGT | 57 |
| R | ACACAGGAATCTTCACAAATGGAAC | ||
| Periostin | F | CATTCAAGGCAGTCTTCAGCC | 56 |
| R | TTTGCAGGTGTGTCTTTTTGC | ||
| MMP-1 | F | TCAGCATGCTTAGCCTTCCT | 58 |
| R | TAGCTTGGACGTCTTCACCC | ||
| MMP-2 | F | GTTATGAGACCCTGAGCCCG | 57 |
| R | CCTTGGGGCAGCCATAGAAA | ||
| MMP-3 | F | TCATGAACTTGGCCACTCCC | 56 |
| R | AACAAGACTTCTCCCCGCAG | ||
| MMP-9 | F | GATCCCCAGAGCGTTACTCG | 58 |
| R | GTTGTGGAAACTCACACGCC | ||
| ADAMTS-4 | F | CCTCGAGACCAGTGCAAACT | 57 |
| R | TGACCACATCGCTGTATCCG | ||
| ADAMTS-5 | F | ATGCACTTCAGCCACGATCA | 56 |
| R | CACACATTTCCCTTGCAGGC | ||
| GAPDH | F | AGTGCCAGCCTCGTCTCATA | 58 |
| R | GATGGTGATGGGTTTCCCGT |