A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic Factors

07 September 2020 : Animal Research

Cheng Zhou^1BCDEF, Chuan Ye^2BCDF, Chen Zhao^1BCD, Junyi Liao^1BCF, Yuwan Li^1BC, Hong Chen^1BF, Wei Huang^1ADEG*

DOI: 10.12659/MSM.924666

Med Sci Monit 2020; 26:e924666

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Table 1 Specific primers for the RT-PCR assay amplifying RUNX-2, OCN, OPN, OSX, and BMP9.

Genes		Sequences (5′-3′)
RUNX-2	Forward	TTCAAGGTTGTAGCCCTCGG
RUNX-2	Reverse	TGAAACTCTTGCCTCGTCCG
OCN	Forward	ATGGCACCACCGTTTAGG
OCN	Reverse	GTGCCGTCCATACTTTCG
OPN	Forward	AAGGACCAACTACAACCA
OPN	Reverse	CATCTGAGTGTTTCGTGT
OSX	Forward	ATGCCAATGACTACCCACCC
OSX	Reverse	ATGGATGCCCGCCTTGTA
BMP9	Forward	GAGTGCCGTGAAGCGTTGG
BMP9	Reverse	GTCCCATTGCTGCGGTCA
GAPDH	Forward	GGCAAGTTCAACGGCACAG
GAPDH	Reverse	CGCCAGTAGACTCCACGACAT
RUNX2 – runt-related transcription factor 2; OCN – osteocalcin; OPN – osteopontin; OSX – osterix; BMP9 – bone morphogenetic protein.

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