07 September 2020: Animal Study
A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic Factors
Cheng Zhou 1BCDEF , Chuan Ye 2BCDF , Chen Zhao 1BCD , Junyi Liao 1BCF , Yuwan Li 1BC , Hong Chen 1BF , Wei Huang 1ADEG*DOI: 10.12659/MSM.924666
Med Sci Monit 2020; 26:e924666
Table 1 Specific primers for the RT-PCR assay amplifying RUNX-2, OCN, OPN, OSX, and BMP9.
| Genes | Sequences (5′-3′) | |
|---|---|---|
| RUNX-2 | Forward | TTCAAGGTTGTAGCCCTCGG |
| Reverse | TGAAACTCTTGCCTCGTCCG | |
| OCN | Forward | ATGGCACCACCGTTTAGG |
| Reverse | GTGCCGTCCATACTTTCG | |
| OPN | Forward | AAGGACCAACTACAACCA |
| Reverse | CATCTGAGTGTTTCGTGT | |
| OSX | Forward | ATGCCAATGACTACCCACCC |
| Reverse | ATGGATGCCCGCCTTGTA | |
| BMP9 | Forward | GAGTGCCGTGAAGCGTTGG |
| Reverse | GTCCCATTGCTGCGGTCA | |
| GAPDH | Forward | GGCAAGTTCAACGGCACAG |
| Reverse | CGCCAGTAGACTCCACGACAT | |
| RUNX2 – runt-related transcription factor 2; OCN – osteocalcin; OPN – osteopontin; OSX – osterix; BMP9 – bone morphogenetic protein. | ||