09 October 2020>: Lab/In Vitro Research
Morphological and Biochemical Properties of Human Astrocytes, Microglia, Glioma, and Glioblastoma Cells Using Fourier Transform Infrared Spectroscopy
Dongsheng Kong 1BEF* , Wenyu Peng 2CDF* , Rui Zong 1ABG* , Gangqiang Cui 3BCD , Xinguang Yu 1AG*DOI: 10.12659/MSM.925754
Med Sci Monit 2020; 26:e925754
Table 1 Assignment of the most prominent absorption bands.
Peak ID | Band/cm−1 | Assignment |
---|---|---|
1 | 965–980 | C-C, PO-stretch: DNA, RNA, phospholipids |
2 | 1039 | C-O-H deformation: glycogen |
3 | 1070–1150 | C-O-C and C-C stretch: carbohydrates, lipids, glycolipids |
4 | 1083–1085 | PO-symmetric stretch: nucleic acids in DNA and RNA |
5 | 1172 | C-O (H) stretch: carbohydrates |
6 | 1237–1244 | PO-asymmetric stretch: nucleic acids, lipids |
7 | 1277 | C-H/N-H deformation: amide III, components of proteins |
8 | 1393–1402 | -COO-symmetric stretch: fatty acids, amino acids |
9 | 1450–1454 | CH, CH deformation: mainly lipids, proteins |
10 | 1538–1546 | N-H bend (60%), C-N stretch (40%) and C-C stretch: amide II, peptide, proteins (α-helix, β-sheets, turn and random coil) |
11 | 1600–1706 | C=O stretch (76%), C-N stretch (14%), CNN (10%): amide I, proteins (α-helix, β-sheets, turn and random coil) |
12 | 1746 | C=O stretch: esters (lipids, phospholipids) |
13 | 1700–1799 | C=O deformation: lipids |
14 | 2844–2860 | -CH symmetric stretch: lipids |
15 | 2892–2924 | -CH symmetric stretch: lipids |
16 | 2953–2994 | -CH asymmetric stretch: lipids |
17 | 3099–3296 | -NH symmetric stretch: amide B, peptides, proteins |
18 | 3327–3337 | -NH asymmetric stretch: amide A, peptides, proteins |