03 November 2020 : Laboratory Research
Role of Sevoflurane on Natural Killer Group 2, Member D-Mediated Immune Response in Non-Small-Cell Lung Cancer: An In Vitro Study
Soeun Jeon12ABCDEFG, Hae-Kyu Kim1DE, Jae-Young Kwon1DF, Seung-Hoon Baek1DE, Hyun-Su Ri3BC, Ho Jung Choi45CD, Hae-Ryung Cho45BD, Young Shin Lee45BD, Joo-Young Kim6BE, Jinsil Kim1BF, Jaeho Bae45ACDEF*, Hyeon-Jeong Lee17ACDEFDOI: 10.12659/MSM.926395
Med Sci Monit 2020; 26:e926395
Table 1 List of primer used in multiplex RT-PCR.
| Name | Polarity | Sequence (5′→3′) | Amplicon length (bp) |
|---|---|---|---|
| MICA | Sense | TTGAGCCGCTGAGAGGGTGGC | 460 |
| Anti-sense | GGGAGAGGAAGAGCTCCCCATC | ||
| MICB | Sense | GCCCCCTGACCCCTTGTTCC | 358 |
| Anti-sense | GGGCTGGTCAACTTGGCGAAA | ||
| ULBP1 | Sense | TGGCTGGTCCCGGGCAGGAT | 266 |
| Anti-sense | GAATGTCAAGCAGTTGCCCTTTAAGGAAA | ||
| ULBP2 | Sense | TCAAACTCGCCCTTCTGTCTGGC | 194 |
| Anti-sense | GCAGGAATTCCATCAGGTAGCACCA | ||
| ULBP3 | Sense | AGGTCTTATCTATGGGTCACCTAGAAG | 132 |
| Anti-sense | TGAAATCCTCCAGCTCAGTGTCAGC | ||
| MMP1 | Sense | AGACAAAGGCAAGTTGAAAAGCGGA | 195 |
| Anti-sense | TTGCTCCCAGCGAGGGTTCC | ||
| MMP2 | Sense | ACGGACTCCTGGCTCATGCC | 305 |
| Anti-sense | CTGTCCTTCAGCGTTGCCGC | ||
| MMP9 | Sense | CGACCCGAGCTGACTCGACG | 390 |
| Anti-sense | GCGGTGTGGTGGTGGTTGGA | ||
| RPL19 | Sense | ATGCTCAGGCTTCAGAAGAGGCTCG | 550 |
| Anti-sense | TGATGATCTCCTCCTTCTTGGCCTG | ||
| ACTB | Sense | TCCATCCTGGCCTCGCTGTC | 93 |
| Anti-sense | GCATTTGCGGTGGACGATGG | ||
| RT-PCR – reverse transcriptase-polymerase chain reaction; MICA/B – MHC class I chain-related molecules A/B; ULBP – UL16-binding proteins; MMP – matrix metallopeptidase; RPL19 – ribosomal protein L19; ACTB – β-actin. | |||