16 November 2020 : Laboratory Research
Construction of a Mex3c Gene-Deficient Mouse Model to Study C-FOS Expression in Hypothalamic Nuclei and Observe Morphological Differences in Embryonic Neural Tube Development
Yong Du12ABDE*, Quan Huo3BCEF, Ting Li3BCD, Dongjun Sun3BEF, Ting Sun4BCD, Zhiguo Lu3BEFDOI: 10.12659/MSM.927334
Med Sci Monit 2020; 26:e927334
Table 1 PCR mixture.
| Component | ×1 |
| ddHO | 9.5 μl |
| Product primer F1 | 1.0 μl |
| Reverse primer R1 | 1.0 μl |
| Premix Taq | 12.5 μl |
| DNA | 1.0 μl |