16 November 2020 : Laboratory Research
Construction of a Mex3c Gene-Deficient Mouse Model to Study C-FOS Expression in Hypothalamic Nuclei and Observe Morphological Differences in Embryonic Neural Tube Development
Yong Du12ABDE*, Quan Huo3BCEF, Ting Li3BCD, Dongjun Sun3BEF, Ting Sun4BCD, Zhiguo Lu3BEFDOI: 10.12659/MSM.927334
Med Sci Monit 2020; 26:e927334
Table 2 DNA cycling condition.
| Step | Temp | Time | Cycles |
|---|---|---|---|
| Initial denaturation | 94°C | 3 min | |
| Denaturation | 94°C | 30 s | 35× |
| Annealing | 52°C | 35 s | |
| Extension | 72°C | 35 s | |
| Additional extension | 72°C | 5 min |