20 December 2020: Animal Study
Effect of Asthma on Erectile Dysfunction in Rats as Determined by Biological Network Analysis
Jisheng Wang 12ABCDEF* , Xuefeng Gong 3ABCDEF* , Sheng Deng 12ABCDEF* , Fanchao Meng 12CDE , Hengheng Dai 12DEF , Binghao Bao 12CD , Junlong Feng 12BF , Haisong Li 2AFG* , Bin Wang 2AFG*DOI: 10.12659/MSM.927491
Med Sci Monit 2020; 26:e927491
Table 1 Specific primer information. Primers were purchased from Invitrogen Biotechnology Co. Ltd. (SJ, CN) and tested by Beijing Huaying Biotechnology Research Institute.
| GeneBank | Primer name | Primer sequence (5′–3′) | Fragment length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| NM 031144.3 | β-Actin 1F | CCTCACTGTCCACCTTCCA | 120 | 66.0 |
| β-Actin 1R | GGGTGTAAAACGCAGCTCA | 65.0 | ||
| XM 021075147.1 | INS 1F | TCTGTCAACTTTGCCGACT | 114 | 64.3 |
| INS 1R | AACCGTGTCTTCGTCCAG | 64.4 | ||
| XM 021075147.1 | IL6 1F | CTCACGCACCGATGTCT | 109 | 64.2 |
| IL6 1R | AGGCTGTGGGCTCAATC | 64.3 | ||
| NM 134326.2 | ALB 1F | GCTGCTGACTTTGTTGAGG | 108 | 64 |
| ALB 1R | GTAATCGGGGTGCCTTCT | 63.9 | ||
| NM 012675.3 | TNF 1F | CAGCCAGGAGGGAGAAC | 93 | 63.9 |
| TNF 1R | GTATGAGAGGGACGGAACC | 63.9 | ||
| NM 00110333.2 | VEGFA 1F | CATCAAGCTCTCTCCTCCA | 91 | 63.2 |
| VEGFA 1R | GGCCTCTTCTTCCACCA | 63.5 |