20 December 2020 : Animal Research
Effect of Asthma on Erectile Dysfunction in Rats as Determined by Biological Network Analysis
Jisheng Wang12ABCDEF, Xuefeng Gong3ABCDEF, Sheng Deng12ABCDEF, Fanchao Meng12CDE, Hengheng Dai12DEF, Binghao Bao12CD, Junlong Feng12BF, Haisong Li2AFG*, Bin Wang2AFGDOI: 10.12659/MSM.927491
Med Sci Monit 2020; 26:e927491
Table 1 Specific primer information. Primers were purchased from Invitrogen Biotechnology Co. Ltd. (SJ, CN) and tested by Beijing Huaying Biotechnology Research Institute.
| GeneBank | Primer name | Primer sequence (5′–3′) | Fragment length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| NM 031144.3 | β-Actin 1F | CCTCACTGTCCACCTTCCA | 120 | 66.0 |
| β-Actin 1R | GGGTGTAAAACGCAGCTCA | 65.0 | ||
| XM 021075147.1 | INS 1F | TCTGTCAACTTTGCCGACT | 114 | 64.3 |
| INS 1R | AACCGTGTCTTCGTCCAG | 64.4 | ||
| XM 021075147.1 | IL6 1F | CTCACGCACCGATGTCT | 109 | 64.2 |
| IL6 1R | AGGCTGTGGGCTCAATC | 64.3 | ||
| NM 134326.2 | ALB 1F | GCTGCTGACTTTGTTGAGG | 108 | 64 |
| ALB 1R | GTAATCGGGGTGCCTTCT | 63.9 | ||
| NM 012675.3 | TNF 1F | CAGCCAGGAGGGAGAAC | 93 | 63.9 |
| TNF 1R | GTATGAGAGGGACGGAACC | 63.9 | ||
| NM 00110333.2 | VEGFA 1F | CATCAAGCTCTCTCCTCCA | 91 | 63.2 |
| VEGFA 1R | GGCCTCTTCTTCCACCA | 63.5 |