Mechanical Stress Affects Circadian Rhythm in Skeletal Muscle (C2C12 Myoblasts) by Reducing Per/Cry Gene Expression and Increasing Bmal1 Gene Expression

Mengjia Wang; Da Yu; Lichun Zheng; Bing Hong; Houxuan Li; Xiaobei Hu; Kun Zhang; Yongbin Mou

doi:10.12659/MSM.928359

14 January 2021 : Laboratory Research

Mechanical Stress Affects Circadian Rhythm in Skeletal Muscle (C2C12 Myoblasts) by Reducing Per/Cry Gene Expression and Increasing Bmal1 Gene Expression

Mengjia Wang^1BCDEF, Da Yu^2BCDF, Lichun Zheng^1BCDF, Bing Hong^1BCD, Houxuan Li^1BCD, Xiaobei Hu^1BC, Kun Zhang^1BF, Yongbin Mou^1ADEG*

DOI: 10.12659/MSM.928359

Med Sci Monit 2021; 27:e928359

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Table 1 The primers for the PCR assay.

Genes		Primers (5′-3′)
	Forward	AGGTCGGTGTGAACGGATTTG
	Reverse	TGTAGACCATGTAGTTGAGGTCA
	Forward	CCTCCTTGCTACAGGTACAT
	Reverse	GGCACATTTACGCTTGGTT
	Forward	GCTGGGCTATGCTGTTTG
	Reverse	CTGCTCCAGGGATTTCAT
	Forward	TGGGAACATCAGGCTATG
	Reverse	TGAGTCTGAAGCCATATCC
	Forward	CTGGGCAACCCCTCTTCA
	Reverse	GCCTTTCCTCTTGCGATTG

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Mechanical Stress Affects Circadian Rhythm in Skeletal Muscle (C2C12 Myoblasts) by Reducing Per/Cry Gene Expression and Increasing Bmal1 Gene Expression

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