29 May 2021 : Laboratory Research
Silencing Ribosomal Protein L22 Promotes Proliferation and Migration, and Inhibits Apoptosis of Gastric Cancer Cells by Regulating the Murine Double Minute 2-Protein 53 (MDM2-p53) Signaling Pathway
Zhenqing Sun1ABCE, Zhigang Qiu1ABCE, Zhengkun Wang1DE, Honghui Chi2DE, Peipei Shan3CE*DOI: 10.12659/MSM.928375
Med Sci Monit 2021; 27:e928375
Table 1 Primer sequences used in the quantitative real-time polymerase chain reaction.
| Name of primer | Sequences (5′-3′) |
|---|---|
| RPL22-F | CATGCCACTTAGGCCATGACT |
| RPL22-R | TGGTAGCCCCTTTCAGTTGTCTA |
| GAPDH-F | GGAGCGAGATCCCTCCAAAAT |
| GAPDH-R | GGCTGTTGTCATACTTCTCATGG |
| si-RPL22-1-F | GCAUUAGAAUUUACGGUGU |
| si-RPL22-1-R | UGAGGAGGUCAACUUCAUC |
| si-RPL22-2-F | GUCAAUAAAAUGCGGGUUU |
| si-RPL22-2-R | AUUAUCCUUUGGAUUCCCG |
| si-NC-F | UUCUCCGAACGUGUCACGU |
| si-NC-R | ACGUGACACGUUCGGAGAA |
| F – forward; GAPDH – glyceraldehyde-3-phosphate dehydrogenase; NC – negative control; R – reverse; RPL22 – ribosomal protein L22; si – short interfering RNA. | |