03 March 2021 : Animal Research
A Network Pharmacology Study of the Molecular Mechanisms of Hypericum japonicum in the Treatment of Cholestatic Hepatitis with Validation in an Alpha-Naphthylisothiocyanate (ANIT) Hepatotoxicity Rat Model
Sen Ling Feng1ACE, Jing Zhang2BCEF, Hongliu Jin1BC, Wen Ting Zhu1BC, Zhongwen Yuan1ABCDEFG*DOI: 10.12659/MSM.928402
Med Sci Monit 2021; 27:e928402
Table 1 Primers for real-time reverse transcription-polymerase chain reaction (RT-PCR).
| Gene | Primer sequences (5′-3′) |
|---|---|
| GAPDH | CCTCGTCCCGTAGACAAAATG |
| TGAGGTCAATGAAGGGGTCGT | |
| PTGS2 | CTGATGACTGCCCAACTCCC |
| CTGGGCAAAGAATGCGAACA | |
| BCL2 | TTGTGGCCTTCTTTGAGTTCG |
| GCATCCCAGCCTCCGTTAT | |
| FXR | TGCCTGTGACAAAGAAGCCG |
| CATTCAGCCAACATTCCCATC | |
| CYP7A1 | GGCATCTCAAGCAAACACCAT |
| GCTGTGCGGATATTCAAGGAT | |
| IL-1β | TCAAATCTCGCAGCAGCACATC |
| CGTCACACACCAGCAGGTTATC | |
| TNF-α | CCCTCACACTCACAAACCACC |
| CTTTGAGATCCATGCCGTTG |