12 March 2021 : Laboratory Research
Citrus Alkaline Extracts Inhibit Senescence of A549 Cells to Alleviate Pulmonary Fibrosis via the β-Catenin/P53 Pathway
Di Han1BDE, Yong Xu1CE, Wen-Pan Peng1BD, Fanchao Feng12AD, Zhichao Wang1BD, Cheng Gu1BD, Xianmei Zhou12AG*DOI: 10.12659/MSM.928547
Med Sci Monit 2021; 27:e928547
Table 1 Primer sequences qRT-PCR.
| Gene | Forward primer | Reveres primer |
|---|---|---|
| GAPDH | GGTTGTCTCCTGCGACTTCA | TGGTCCAGGGTTTCTTACTCC |
| PDGF-a | TTCGCAGGAAGAGAAGTATTGAGGAAG | CCGTGAAGGCTGGCACTTGAC |
| PDGF-b | TCTGCTGCTACCTGCGTCTGG | CATCTTCATCTACGGAGTCTCTGTGC |
| MMP-7 | CCACTCACCTGCTGCTACTCATTC | CTGCTGCTGGTGATCCTCTTGTAG |
| CTGF | AGCTGCCTACCGACTGGAAGAC | GGTGGTTCTGTGCGGTGTGC |
| TNF-α | GCGACGTGGAACTGGCAGAAG | GAATGAGAAGAGGCTGAGACATAGGC |
| CyclinD1 | TGGATTGATTCGAAATCTTGCC | GAACAAGCAACTGAACTAGTCG |
| GSK-3β | AGGAGAACCCAATGTTTCGTAT | ATCCCCTGGAAATATTGGTTGT |
| Collagen I | CGGCCCTGCTGGAAACCCTC | GGGAGCACCACGTTCACCGG |
| Collagen III | CCTTCGACTTCTCTCCAGCC | TTTCGTGCAACCATCCTCCA |
| α-SMA | CTTTGGCTTGGCTTGTCAGG | CGGACAGGAATTGAAGCGGA |
| β-catenin | TGAGGACAAGCCACAAGATTAC | TCCACCAGAGTGAAAAGAACG |