23 June 2021 : Clinical Research
A Comparison of Candida Detection in Sputum by the Conventional Culture and Fluorescent Polymerase Chain Reaction Methods
Xiaoqun Xu12BD, Huoyang Lv3AE, Fengwei Zhang12DF, Houyong Zhu4CDE, Long Cai12AE*DOI: 10.12659/MSM.930293
Med Sci Monit 2021; 27:e930293
Table 1 The sputum DNA extraction kit.
| Sequence | Component | Storage conditions | Specification and quantity |
|---|---|---|---|
| 1 | Sputum digestive fluid | Room temperature | 50 ml |
| 2 | Sample cleaning solution | Room temperature | 40 ml |
| 3 | Sample treatment solution A | Room temperature | 6 ml |
| 4 | DNA solid extract | Room temperature | 20 Tubes |
| 5 | Sample treatment solution B | Room temperature | 4 ml |
| 6 | DNA ourification column | Room temperature | 20 Groups |
| 7 | Flushing fluid | Room temperature | 7.5 ml |
| 8 | DNA eluent | Room temperature | 1 ml |