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23 June 2021: Clinical Research

A Comparison of Detection in Sputum by the Conventional Culture and Fluorescent Polymerase Chain Reaction Methods

Xiaoqun Xu 12BD* , Huoyang Lv 3AE* , Fengwei Zhang 12DF , Houyong Zhu 4CDE* , Long Cai 12AE*

DOI: 10.12659/MSM.930293

Med Sci Monit 2021; 27:e930293

Table 2 The universal nucleic acid detection kit.

KitComponentsStorage conditionsSpecifications and quantitiesMain components
A-boxPrimers−20°C22 μL ×1 TubeUpstream and downstream primers for detection
Probe−20°C, protect form light11 μL×1 TubeProbe for detection
Negative control−20°C15 μL ×1 TubePlasmid containing unrelated gene
Positive control−20°C15 μL ×1 TubePlasmid Containing Target Gene
B-boxPCR reaction solution2–8°C, freeze prohibited220 μL ×1 TubedNTP (containing dUTP), Taq enzyme, UNG enzyme, reference fluorescence, MgCl2, buffer

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750