A Comparison of Candida Detection in Sputum by the Conventional Culture and Fluorescent Polymerase Chain Reaction Methods

23 June 2021 : Clinical Research

Xiaoqun Xu^12BD, Huoyang Lv^3AE, Fengwei Zhang^12DF, Houyong Zhu^4CDE, Long Cai^12AE*

DOI: 10.12659/MSM.930293

Med Sci Monit 2021; 27:e930293

Background Material and Methods Results Discussion Conclusions References

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Table 2 The universal nucleic acid detection kit.

Kit	Components	Storage conditions	Specifications and quantities	Main components
A-box	Primers	−20°C	22 μL ×1 Tube	Upstream and downstream primers for detection
	Probe	−20°C, protect form light	11 μL×1 Tube	Probe for detection
	Negative control	−20°C	15 μL ×1 Tube	Plasmid containing unrelated gene
	Positive control	−20°C	15 μL ×1 Tube	Plasmid Containing Target Gene
B-box	PCR reaction solution	2–8°C, freeze prohibited	220 μL ×1 Tube	dNTP (containing dUTP), Taq enzyme, UNG enzyme, reference fluorescence, MgCl2, buffer

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