29 April 2021 : Animal Research
miR-1283 Contributes to Endoplasmic Reticulum Stress in the Development of Hypertension Through the Activating Transcription Factor-4 (ATF4)/C/EBP-Homologous Protein (CHOP) Signaling Pathway
Weihao Chen1ABC, Tianhao Liu1EF*, Qiuer Liang1F, Xudong Chen1BC, Wencong Tao1B, Meixia Fang1AE, Ya Xiao1AE, Liguo Chen1AEGDOI: 10.12659/MSM.930552
Med Sci Monit 2021; 27:e930552
Table 1 Primer sequences of mouse and human aortic cells.
| Gene | Sample | Primer |
|---|---|---|
| ATF4 | Mouse | F: 5′-CAAAACAAGACAGCAGCCACTA-3′ |
| R: 5′-CTTCTTCCCCCTTGCCTTAC-3′ | ||
| HAEC | F: 5′-TGCCCGTCCCAAACCTTAC-3′ | |
| R: 5′-TGCTCCGCCCTCTTCTTCT-3′ | ||
| BCL-X | Mouse | F: 5′-GGAGAGCGTTCAGTGATC-3′ |
| R: 5′-CAATGGTGGCTGAAGAGA-3′ | ||
| HAEC | F: 5′-GGGCATTCAGTGACCTGACA-3′ | |
| R: 5′-ATGCGACCCCAGTTTACCC-3′ | ||
| BCL-2 | Mouse | F: 5′-AGGGGGAAACACCAGAATC-3′ |
| R: 5′-GGTAGCGACGAGAGAAGTCA-3′ | ||
| HAEC | F: 5′-AACATCGCCCTGTGGATGAC-3′ | |
| R: 5′-AGAGTCTTCAGAGACAGCCAGGAG-3′ | ||
| BID | Mouse | F: 5′-TGGAGAAGGACCTGATAACC-3′ |
| R: 5′-CTTGTAGGCAGTGACGATGA-3′ | ||
| HAEC | F: 5′-AATGGCCTTCATATCATCCACACA-3′ | |
| R: 5′-GGGAACCTGCACAGTGGAAATAA-3′ | ||
| Caspase-3 | Mouse | F: 5′-GTCCCACTGTCTGTCTCA-3′ |
| R: 5′-GTCTCGCTCTACTGTAAG-3′ | ||
| HAEC | F: 5′-GACTCTGGAATATCCCTGGACAACA-3′ | |
| R: 5′-AGGTTTGCTGCATCGACATCTG-3′ | ||
| CHOP | Mouse | F: 5′-CTCGCTCTCCAGATTCCAGT-3′ |
| R: 5′-CTGCTCCTTCTCCTTCATGC-3′ | ||
| HAEC | F: 5′-TCTAAGGCACTGAGCGTATCATGT-3′ | |
| R: 5′-TTTCAGGTGTGGTGATGTATGAAG-3′ | ||
| BIM | Mouse | F: 5′-GGCCAAGCAACCTTCTGACT-3′ |
| R: 5′-TGCCTTCTCCATACCAGACG-3′ | ||
| HAEC | F: 5′-CATCATCGCGGTATTCGGTTC-3′ | |
| R: 5′-AAGGTTGCTTTGCCATTTGGTC-3′ | ||
| GAPDH | Mouse | F: 5′-tggccgtggggctgcccag-3′ |
| R: 5′-ggaaggccatgccagtgagc-3′ | ||
| HAEC | F: 5′-GCACCGTCAAGGCTGAGAAC-3′ | |
| R: 5′-TGGTGAAGACGCCAGTGGA-3′ |