05 October 2021 : Clinical Research
miRNA Profiling of Hungarian Regressive Wilms’ Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)
Gergely Buglyó1ABCDEF*, Zsófia Magyar2ABCD, Éva Romicsné Görbe
2AB, Rita Bánusz3AB, Monika Csóka 3B, Tamás Micsik4BDE, Márta Mezei1BC, Jaxi Ayman Shawky Yani1BC, Péter Varga2A, Zoltán Sápi4A, Bálint Nagy1ACD
DOI: 10.12659/MSM.932731
Med Sci Monit 2021; 27:e932731
Table 1 Data on enrolled patients and FFPE samples with obtained miRNA concentrations.
| Patient ID | Age (in years) | Sex | Age of FFPE sample (in years) | Histology | miRNA concentration in tumor | miRNA concentration in control |
|---|---|---|---|---|---|---|
| 1 | 1 | F | 6 | Stromal | 57.2 ng/μl | >75 ng/μl |
| 2 | 2 | F | 6 | Triphasic | 18.1 ng/μl | >75 ng/μl |
| 3 | 3 | M | 6 | Blastemal | 16.7 ng/μl | 16.5 ng/μl |
| 4 | 7 | M | 9 | Triphasic | >75 ng/μl | 5.7 ng/μl |
| 5 | 3 | F | 7 | Blastemal | 28 ng/μl | >75 ng/μl |
| 6 | 3 | M | 8 | Blastemal | 15.5 ng/μl | >75 ng/μl |
| 7 | 3 | F | 6 | Stromal | 31.7 ng/μl | 58.5 ng/μl |
| 8 | 3 | F | 5 | Triphasic* | >75 ng/μl | >75 ng/μl |
| 9 | 7 | F | 7 | ** | – | – |
| 10 | 2 | F | 7 | ** | – | – |
| * Histology was somewhat unusual in this case, as nearly all blastema was necrotic and viable elements in the tumor were mostly stromal. ** These samples were almost completely necrotic: histology could not be assessed. miRNA extraction also failed, probably due to a lack of viable cells, so they were excluded from the rest of the study (see main text). | ||||||