miRNA Profiling of Hungarian Regressive Wilms’ Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

Gergely Buglyó; Zsófia Magyar; Éva Romicsné Görbe; Rita Bánusz; Monika Csóka; Tamás Micsik; Márta Mezei; Jaxi Ayman Shawky Yani; Péter Varga; Zoltán Sápi; Bálint Nagy

doi:10.12659/MSM.932731

05 October 2021 : Clinical Research

miRNA Profiling of Hungarian Regressive Wilms’ Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

Gergely Buglyó^1ABCDEF*, Zsófia Magyar^2ABCD, Éva Romicsné Görbe

^2AB, Rita Bánusz^3AB, Monika Csóka

^3B, Tamás Micsik^4BDE, Márta Mezei^1BC, Jaxi Ayman Shawky Yani^1BC, Péter Varga^2A, Zoltán Sápi^4A, Bálint Nagy^1ACD

DOI: 10.12659/MSM.932731

Med Sci Monit 2021; 27:e932731

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Table 2 Ct values and fold changes obtained in the pilot experiment by PCR array from tumor and control samples of Patient 1 using SNORD61 as endogenous control. Log2FC is the logarithm of fold change, defined as the opposite of ΔΔCt.

miRNA	Ct (tumor)	Ct (control)	Log2FC
let-7a-5p	21.05	18.60	−0.84
let-7b-5p	20.92	18.71	−0.6
let-7c	21.82	20.75	0.54
let-7f-5p	23.91	20.44	−1.86
miR-100-5p	21.42	18.59	−1.22
miR-101-3p	24.81	21.91	−1.29
miR-106b-5p	23.96	21.11	−1.24
miR-125a-5p	19.65	17.33	−0.71
miR-125b-5p	17.64	17.47	1.44
miR-126-3p	22.05	18.28	−2.16
miR-126-5p	25.34	21.29	−2.44
miR-128	23.58	25.18	3.21
miR-133a	23.11	28.88	7.38
miR-135a-5p	28.60	24.65	−2.34
miR-135b-5p	29.36	25.02	−2.73
miR-141-3p	26.99	22.43	−2.95
miR-143-3p	22.20	20.00	−0.59
miR-145-5p	19.64	17.82	−0.21
miR-146a-5p	28.24	24.31	−2.32
miR-146b-5p	25.12	21.46	−2.05
miR-148a-3p	23.47	22.49	0.63
miR-15a-5p	25.05	23.11	−0.33
miR-15b-5p	25.04	23.33	−0.1
miR-16-5p	21.62	19.07	−0.94
miR-17-5p	25.03	22.01	−1.41
miR-17-3p	27.95	25.69	−0.65
miR-181a-5p	23.26	21.66	0.01
miR-181b-5p	23.79	22.08	−0.1
miR-182-5p	27.40	26.37	0.58
miR-183-5p	28.48	26.96	0.09
miR-184	33.80	28.48	−3.71
miR-194-5p	27.93	20.32	−6
miR-195-5p	22.37	19.51	−1.25
miR-196a-5p	24.65	23.78	0.74
miR-19b-3p	23.49	20.77	−1.11
miR-200b-3p	25.74	19.80	−4.33
miR-200c-3	25.04	21.04	−2.39
miR-203a	28.61	25.02	−1.98
miR-205-5p	23.48	25.77	3.9
miR-20a-5p	24.07	20.23	−2.23
miR-20b-5p	25.99	22.22	−2.16
miR-21-5p	20.74	16.63	−2.5
miR-218-5p	27.52	26.35	0.44
miR-22-3p	23.11	19.70	−1.8
miR-221-3p	24.41	21.75	−1.05
miR-222-3p	24.05	21.39	−1.05
miR-223-3p	24.95	21.68	−1.66
miR-224-5p	27.53	26.40	0.48
miR-23b-3p	21.68	19.13	−0.94
miR-24-3p	21.11	18.56	−0.94
miR-25-3p	24.30	22.27	−0.42
miR-26a-5p	20.34	17.46	−1.27
miR-26b-5p	22.52	19.59	−1.32
miR-27a-3p	22.47	19.81	−1.05
miR-27b-3p	23.30	20.33	−1.36
miR-296-5p	24.71	24.96	1.86
miR-29b-3p	26.85	21.37	−3.87
miR-30c-5p	22.68	18.11	−2.96
miR-31-5p	28.71	24.20	−2.9
miR-3163	33.56	32.99	1.04
miR-32-5p	28.48	25.13	−1.74
miR-330-3p	28.23	26.62	0
miR-331-3p	24.51	22.23	−0.67
miR-34a-5p	23.04	21.36	−0.07
miR-34b-3p	28.91	26.57	−0.73
miR-34c-5p	29.21	29.16	1.56
miR-361-5p	25.59	23.75	−0.23
miR-365a-3p	23.53	22.01	0.09
miR-3662	32.59	31.08	0.1
miR-3666	32.12	31.06	0.55
miR-374b-5p	25.48	22.30	−1.57
miR-375	28.95	27.11	−0.23
miR-425-5p	25.98	23.66	−0.71
miR-449a	29.51	27.95	0.05
miR-455-5p	26.97	24.85	−0.51
miR-494	21.82	20.65	0.44
miR-616-3p	30.85	30.42	1.18
miR-7-5p	29.74	26.86	−1.27
miR-9-3p	32.29	28.27	−2.41
miR-92a-3p	20.62	19.05	0.04
miR-93-5p	25.11	21.95	−1.55
miR-96-5p	28.85	27.27	0.03
miR-99a-5p	20.37	19.88	1.12
miR-99b-5p	22.72	20.34	−0.77
SNORD61	19.57	17.96

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miRNA Profiling of Hungarian Regressive Wilms’ Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

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