07 May 2022 : Animal Research
Characterization of Inflammatory Factors and T Cell Subpopulations in a Murine Model of Kawasaki Disease Induced by Candida albicans Cell Wall Extracts (CAWS)
Huifang Xu1ABG*, Jing Weng1BCE, Quan Bao1CE, Fengyan Nie
1D, Yuqiang Jiang 1E, Cong Xiao1F
DOI: 10.12659/MSM.936355
Med Sci Monit 2022; 28:e936355
Table 1 Primer sequences of RT-qPCR.
| Target genes | Primer sequences (5′-3′) |
|---|---|
| IL-1β | F: GAAATGCCACCTTTTGACAGTGR: TGGATGCTCTCATCAGGACAG |
| IL-6 | F: CCAAGAGGTGAGTGCTTCCCR: CTGTTGTTCAGACTCTCTCCCT |
| IL-10 | F: GCTCTTACTGACTGGCATGAGR: CGCAGCTCTAGGAGCATGTG |
| G-CSF | F: GCACTATGGTCAGGACGAGAGR: GGGGAAATACCCGATAGAGCC |
| TNF-α | F: GACGTGGAACTGGCAGAAGAGR: TTGGTGGTTTGTGAGTGTGAG |
| MIP-1α | F: TGTACCATGACACTCTGCAACR: CAACGATGAATTGGCGTGGAA |
| RANTES | F: TTTGCCTACCTCTCCCTCGR: CGACTGCAAGATTGGAGCACT |
| MCP-1 | F: TAAAAACCTGGATCGGAACCAAAR: GCATTAGCTTCAGATTTACGGGT |
| VCAM-1 | F: TTGGGAGCCTCAACGGTACTR: GCAATCGTTTTGTATTCAGGGGA |
| ICAM-1 | F: GTGATGCTCAGGTATCCATCCAR: CACAGTTCTCAAAGCACAGCG |
| E-selectin | F: ATGAAGCCAGTGCATACTGTCR: CGGTGAATGTTTCAGATTGGAGT |
| GAPDH | F: TGGCCTTCCGTGTTCCTACR: GAGTTGCTGTTGAAGTCGCA |