02 July 2023: Review Articles
Advancements in Zebrafish Models for Breast Cancer Research: Unveiling Biomarkers, Targeted Therapies, and Personalized Medicine
Anna Wawruszak ABCDEFG* , Estera Okoń ABCDEF , Karolina Dudziak ABCDEFGDOI: 10.12659/MSM.940550
Med Sci Monit 2023; 29:e940550
Background
Zebrafish Breast Cancer In Vivo Models
Breast Cancer Patient-Derived Xenografts
Troubleshooting In Vivo Models
Epithelial-Mesenchymal Transition (EMT) in Tumor Metastasis
Modeling Breast Cancer Metastasis with Zebrafish
Importance of TGF-β in the Process of Metastasis
Role of PTEN in Invasion and Migration of Cancer Cells
Role of CXCR4-CXCL12 Chemokine Signaling Axis in Cancer Cell Migration
Metastatic Capacity of Circulating Tumor Cells
Study of Effect of the Microenvironment on Breast Cancer Cells
Zebrafish as a Preclinical Model for Drug Screening
Monoclonal Antibodies Studies in Zebrafish Model
Synthetic Drugs Studies in Zebrafish Model
Natural Products Studies in Zebrafish Model
Future Directions
Conclusions
References
Table 1 Zebrafish (ZF) (Danio reiro) as a tool for breast cancer (BC) drug screening.
| Group of agents | Active agent | Zebrafish xenograft model | Mechanism of action | Ref. |
|---|---|---|---|---|
| Monoclonal antibodies | Olaparib (monotherapy or combination with IR) | Hs578T, MDA-MB-468 and HCC1937 TNBC ZF xenograft models | aberrant mitosis in MDA-MB-468 xenografts. cytotoxicity of olaparib, enhancement of the accumulation of DNA damage, activation of cell-death signaling pathways in the MDA-MB-468 xenografts, mitotic aberrancies, ↓ tumor size, ↓ tumor vessel density in Hs578T xenografts↑ | []32 |
| Bevacizumab | Hs578T and MDA-MB-468 ZF xenograft models | apoptosis,↓ tumor size,↓ vessel density,↓ vessel infiltration in Hs578T xenografts↑ | []71 | |
| Synthetic drugs | Acetin | MDA-MB-231 ZF xenograft model | ↓ number of migratory cells,↓ proliferation | []72 |
| Edelfozine (nanometric emulsion) | MDA-MB-231 ZF xenograft model | ↓ proliferation,regression of the tumor | []73 | |
| 5k | MCF7 ZF xenograft model | ↓ proliferation,↓ angiogenesis,↓ tumor area and volume,↓ number and length of ectopic vessels | []74 | |
| Ru1 and Ru2 | MCF7 ZF xenograft model | ↓ proliferation,↓ tumor growth | []75 | |
| IT1t | MDA-MB-231 ZF xenograft model | ↓ early metastasis | []36 | |
| Natural products | Fangjihuangqi decotion | MDA-MB-231 ZF xenograft model | ↓ tumor growth,↓ number of cancer cells in the abdominal cavity,↓ length of tumor neoplastic lymphatics,↓ number of tumor neovascularization,improvement the abnormal morphology of tumor cells, E-cadherin and ↓ Snail2, TWIST1, ZEB2 and TGFβ1 protein expression↑ | []76 |
| Gomisin 2 | MDA-MB-231 and HCC1806 progenitor TNBC ZF xenograft models | ↓ cell growth,↓ proliferation | []77 | |
| Bakuchiol | MCF7 progenitor luminal ZF xenograft models | apoptosis,↓ metastasis↑ | []78 | |
| Furanodiene | MCF7 luminal and MDA-MB-231 (native and adriamycin-resistant) TNBC ZF xenograft models | Synergistic anti-cancer effect with 5-FU in MDA-MB-231 xenografts,reversion of MDR in adriamycin-resistant MDA-MB-231 cell xenografts, caspase 8- and 9- dependent apoptosis,↓ angiogenesis, ROS production and DNA strand breaks,↓ Pgp protein level↑ | []79 | |
| Jodomycin B | MDA-MB-231 TNBC ZF xenograft model | ↓ viability,↓ proliferation | []80 | |
| ↑ – increase; ↓ – decrease; DNA – deoxyribonucleic acid; IR – ionizing radiation; MDR – multidrug-resistant; Pgp – permeability glycoprotein; ROS – reactive oxygen species; Ru1 – [Ru(bipy)2(C12H8N6-N,N)][CF3SO3]2; Ru2 – [{Ru(bipy)2}2(μ-C12H8N6-N,N)][CF3SO3]4; TGFβ1 – transforming growth factor beta 1; TNBC – triple-negative breast cancer; TWIST1 – twist family bHLH transcription factor 1; ZEB2 – zinc finger E-box binding homeobox 2; ZF – zebrafish. | ||||